A novel motif governs APC-dependent degradation of Drosophila ORC1 in vivo

Marito Araki, Hongtao Yu, Maki Asano

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Regulated degradation plays a key role in setting the level of many factors that govern cell cycle progression. In Drosophila, the largest subunit of the origin recognition complex protein 1 (ORC1) is degraded at the end of M phase and throughout much of G1 by anaphase-promoting complexes (APC) activated by Fzr/Cdh1. We show here that none of the previously identified APC motifs targets ORC1 for degradation. Instead, a novel sequence, the O-box, is necessary and sufficient to direct Fzr/Cdh1-dependent polyubiquitylation in vitro and degradation in vivo. The O-box is similar to but distinct from the well characterized D-box. Finally, we show that O-box motifs in two other proteins, Drosophila Abnormal Spindle and Schizosaccharomyces pombe Cut2, contribute to Cdh1-dependent polyubiquitylation in vitro, suggesting that the O-box may mediate degradation of a variety of cell cycle factors.

Original languageEnglish (US)
Pages (from-to)2458-2465
Number of pages8
JournalGenes and Development
Volume19
Issue number20
DOIs
StatePublished - Oct 15 2005

Keywords

  • APC
  • Cell cycle
  • ORC1
  • Proteolysis
  • Replication

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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