A novel mouse model overexpressing Nocturnin results in decreased fat mass in male mice

Phuong T. Le, Sheila A. Bornstein, Katherine J. Motyl, Li Tian, Jeremy J. Stubblefield, Hee Kyung Hong, Joseph S Takahashi, Carla B Green, Clifford J. Rosen, Anyonya R. Guntur

Research output: Contribution to journalArticle

Abstract

Nocturnin (NOCT) belongs to the Mg2+ dependent Exonucleases, Endonucleases, Phosphatase (EEP) family of enzymes that exhibit various functions in vitro and in vivo. NOCT is known to function as a deadenylase, cleaving poly-A tails from mRNA (messenger RNA) transcripts. Previously, we reported a role for NOCT in regulating bone marrow stromal cell differentiation through its interactions with PPARγ. In this study, we characterized the skeletal and adipose tissue phenotype when we globally overexpressed Noct in vivo. After 12 weeks of Noct overexpression, transgenic male mice had lower fat mass compared to controls, with no significant differences in the skeleton. Based on the presence of a mitochondrial target sequence in NOCT, we determined that mouse NOCT protein localizes to the mitochondria; subsequently, we found that NOCT overexpression led to a significant increase in the preadipocytes ability to utilize oxidative phosphorylation for ATP (adenosine triphosphate) generation. In summary, the effects of NOCT on adipocytes are likely through its novel role as a mediator of mitochondrial function.

Original languageEnglish (US)
Pages (from-to)20228-20239
Number of pages12
JournalJournal of Cellular Physiology
Volume234
Issue number11
DOIs
StatePublished - Nov 1 2019

Fingerprint

Fats
Exonucleases
Messenger RNA
Peroxisome Proliferator-Activated Receptors
Mitochondria
Endonucleases
Oxidative Phosphorylation
nocturnin
Mesenchymal Stromal Cells
Phosphoric Monoester Hydrolases
Adipocytes
Skeleton
Transgenic Mice
Adipose Tissue
Cell Differentiation
Bone
Adenosine Triphosphate
Tissue
Phenotype
Enzymes

Keywords

  • adipogenesis and osteogenesis
  • bone mass
  • Nocturnin

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Le, P. T., Bornstein, S. A., Motyl, K. J., Tian, L., Stubblefield, J. J., Hong, H. K., ... Guntur, A. R. (2019). A novel mouse model overexpressing Nocturnin results in decreased fat mass in male mice. Journal of Cellular Physiology, 234(11), 20228-20239. https://doi.org/10.1002/jcp.28623

A novel mouse model overexpressing Nocturnin results in decreased fat mass in male mice. / Le, Phuong T.; Bornstein, Sheila A.; Motyl, Katherine J.; Tian, Li; Stubblefield, Jeremy J.; Hong, Hee Kyung; Takahashi, Joseph S; Green, Carla B; Rosen, Clifford J.; Guntur, Anyonya R.

In: Journal of Cellular Physiology, Vol. 234, No. 11, 01.11.2019, p. 20228-20239.

Research output: Contribution to journalArticle

Le, PT, Bornstein, SA, Motyl, KJ, Tian, L, Stubblefield, JJ, Hong, HK, Takahashi, JS, Green, CB, Rosen, CJ & Guntur, AR 2019, 'A novel mouse model overexpressing Nocturnin results in decreased fat mass in male mice', Journal of Cellular Physiology, vol. 234, no. 11, pp. 20228-20239. https://doi.org/10.1002/jcp.28623
Le PT, Bornstein SA, Motyl KJ, Tian L, Stubblefield JJ, Hong HK et al. A novel mouse model overexpressing Nocturnin results in decreased fat mass in male mice. Journal of Cellular Physiology. 2019 Nov 1;234(11):20228-20239. https://doi.org/10.1002/jcp.28623
Le, Phuong T. ; Bornstein, Sheila A. ; Motyl, Katherine J. ; Tian, Li ; Stubblefield, Jeremy J. ; Hong, Hee Kyung ; Takahashi, Joseph S ; Green, Carla B ; Rosen, Clifford J. ; Guntur, Anyonya R. / A novel mouse model overexpressing Nocturnin results in decreased fat mass in male mice. In: Journal of Cellular Physiology. 2019 ; Vol. 234, No. 11. pp. 20228-20239.
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