A novel OxyR sensor and regulator of hydrogen peroxide stress with one cysteine residue in Deinococcus radiodurans

Huan Chen, Guangzhi Xu, Ye Zhao, Bing Tian, Huiming Lu, Xiaomin Yu, Zhenjian Xu, Nanjiao Ying, Songnian Hu, Yuejin Hua

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

In bacteria, OxyR is a peroxide sensor and transcription regulator, which can sense the presence of reactive oxygen species and induce antioxidant system. When the cells are exposed to H2O2. OxyR protein is activated via the formation of a disulfide bond between the two conserved cysteine residues (C199 and C208). In Denococcus radiodurans, a previously unreported special characteristic of DrOyR (DR0615) is found with only one conserved cysteine. dr0615 gene mutant is hypersensitive to H2O2, but only a little to ionizing radiation. Site-directed mutagenesis and subsequent in vivo functional binding characteristics of OxyR on DNA. Under oxidant stress, DrOxyR is oxidized to sulfenic acid form, which can be reduced by reducing reagents. In addition, quantitative real-time PCR and global transcription profile results showed that OxyR is not only a transcriptional activator (e.g., kate drb0125), but also a transcriptional repressor (e.g., dps. mntH). Because OxyR regulates Mn and Fe ion transporter genes, Mn/Fe Ion ratio is changed in dr0615 mutant, suggesting that the genes involved in Mn/Fe ion homeostatis, and the genes involved in antioxidant mechanism are highly cooperative under extremely oxidant stress. In conclusion, these findings expand the OxyR family, which could be divided into classes: typical 2-Cys OxyR and 1-Cys OxyR.

Original languageEnglish (US)
Article numbere1602
JournalPLoS One
Volume3
Issue number2
DOIs
StatePublished - Feb 13 2008

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Deinococcus
Deinococcus radiodurans
Hydrogen Peroxide
Cysteine
hydrogen peroxide
cysteine
Genes
Sensors
Transcription
Ions
ions
Oxidants
oxidants
Sulfenic Acids
genes
Antioxidants
antioxidants
mutants
Mutagenesis
disulfide bonds

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

A novel OxyR sensor and regulator of hydrogen peroxide stress with one cysteine residue in Deinococcus radiodurans. / Chen, Huan; Xu, Guangzhi; Zhao, Ye; Tian, Bing; Lu, Huiming; Yu, Xiaomin; Xu, Zhenjian; Ying, Nanjiao; Hu, Songnian; Hua, Yuejin.

In: PLoS One, Vol. 3, No. 2, e1602, 13.02.2008.

Research output: Contribution to journalArticle

Chen, Huan ; Xu, Guangzhi ; Zhao, Ye ; Tian, Bing ; Lu, Huiming ; Yu, Xiaomin ; Xu, Zhenjian ; Ying, Nanjiao ; Hu, Songnian ; Hua, Yuejin. / A novel OxyR sensor and regulator of hydrogen peroxide stress with one cysteine residue in Deinococcus radiodurans. In: PLoS One. 2008 ; Vol. 3, No. 2.
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