A novel polyamine acyltransferase responsible for the accumulation of spermidine conjugates in Arabidopsis seed

Jie Luo, Christine Fuell, Adrian Parr, Lionel Hill, Paul Bailey, Katherine Elliott, Shirley A. Fairhurst, Cathie Martin, Anthony J. Michael

Research output: Contribution to journalArticle

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Abstract

Hydroxycinnamic acid amides are a class of secondary metabolites distributed widely in plants. We have identified two sinapoyl spermidine derivatives, N-((4′-O-glycosyl)-sinapoyl),N′-sinapoylspermidine and N,N′-disinapoylspermidine, which comprise the two major polyamine conjugates that accumulate in Arabidopsis thaliana seed. Using metabolic profiling of knockout mutants to elucidate the functions of members of the BAHD acyltransferase family in Arabidopsis, we have also identified two genes encoding spermidine disinapoyl transferase (SDT) and spermidine dicoumaroyl transferase (SCT) activities. At2g23510, which is expressed mainly in seeds, encodes a spermidine sinapoyl CoA acyltransferase (SDT) that is required for the production of disinapoyl spermidine and its glucoside in Arabidopsis seed. The structurally related BAHD enzyme encoded by At2g25150 is expressed specifically in roots and has spermidine coumaroyl CoA acyltransferase (SCT) activity both in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)318-333
Number of pages16
JournalPlant Cell
Volume21
Issue number1
DOIs
StatePublished - Jan 2009

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Acyltransferases
acyltransferases
Spermidine
Polyamines
spermidine
polyamines
Arabidopsis
Seeds
Transferases
seeds
transferases
Coumaric Acids
knockout mutants
coumaric acids
Glucosides
metabolomics
Coenzyme A
amides
Amides
secondary metabolites

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

A novel polyamine acyltransferase responsible for the accumulation of spermidine conjugates in Arabidopsis seed. / Luo, Jie; Fuell, Christine; Parr, Adrian; Hill, Lionel; Bailey, Paul; Elliott, Katherine; Fairhurst, Shirley A.; Martin, Cathie; Michael, Anthony J.

In: Plant Cell, Vol. 21, No. 1, 01.2009, p. 318-333.

Research output: Contribution to journalArticle

Luo, J, Fuell, C, Parr, A, Hill, L, Bailey, P, Elliott, K, Fairhurst, SA, Martin, C & Michael, AJ 2009, 'A novel polyamine acyltransferase responsible for the accumulation of spermidine conjugates in Arabidopsis seed', Plant Cell, vol. 21, no. 1, pp. 318-333. https://doi.org/10.1105/tpc.108.063511
Luo, Jie ; Fuell, Christine ; Parr, Adrian ; Hill, Lionel ; Bailey, Paul ; Elliott, Katherine ; Fairhurst, Shirley A. ; Martin, Cathie ; Michael, Anthony J. / A novel polyamine acyltransferase responsible for the accumulation of spermidine conjugates in Arabidopsis seed. In: Plant Cell. 2009 ; Vol. 21, No. 1. pp. 318-333.
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