A novel protein fraction from Sesbania grandiflora shows potential anticancer and chemopreventive efficacy, in vitro and in vivo

Krishna P. Laladhas, Vino T. Cheriyan, Vineshkumar T. Puliappadamba, Smitha V. Bava, Rajesh G. Unnithan, Parvathy L. Vijayammal, Ruby John Anto

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We report mechanism-based evidence for the anticancer efficacy of a protein fraction, SF2 (Sesbania fraction 2) isolated from the flower of the medicinal plant, Sesbania grandiflora (S. grandiflora). The fraction was evaluated in two murine ascites tumour cell lines and human cancer cell lines of different origin for its anticancer effect. SF2 inhibited cell proliferation and induced apoptosis as demonstrated by DNA fragmentation and externalization of phosphatidyl serine in Daltons lymphoma ascites (DLA) and colon cancer cells (SW-480). Sensitivity to SF2 in these cells was associated with activation of caspases 3, 8 and 9, poly (ADP-ribose) polymerase cleavage and cytochrome C release which attests apoptosis induced cell death. Mechanistically, SF2 down-regulated phorbol myristate acetate (PMA) induced NF-κB, a transcription factor which controls the expression of genes encoding proteins involved in cell regulation and growth control. Additionally, SF2 also down-regulated anti-apoptotic factors such as Bcl-2, p-Akt and cyclooxygenase-2 induced by the tumour promoter PMA suggestive of a possible explanation for its anticancer effect. In vivo studies using ascites and solid tumour models strongly support in vitro findings as SF2 administration increased the life span and decreased the tumour volume in mice bearing tumour. In vivo toxicological evaluation revealed the pharmacological safety of SF2 and may serve as a potential anticancer drug candidate.

Original languageEnglish (US)
Pages (from-to)636-646
Number of pages11
JournalJournal of Cellular and Molecular Medicine
Volume14
Issue number3
DOIs
StatePublished - Mar 1 2010

Fingerprint

Sesbania
Proteins
Ascites
Tetradecanoylphorbol Acetate
Apoptosis
Neoplasms
Poly(ADP-ribose) Polymerases
In Vitro Techniques
Caspase 9
Caspase 8
Phosphatidylserines
DNA Fragmentation
Cyclooxygenase 2
Cytochromes
Medicinal Plants
Tumor Burden
Tumor Cell Line
Caspase 3
Carcinogens
Colonic Neoplasms

Keywords

  • Anticancer
  • Apoptosis
  • Chemoprevention
  • Sesbania grandiflora

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine

Cite this

A novel protein fraction from Sesbania grandiflora shows potential anticancer and chemopreventive efficacy, in vitro and in vivo. / Laladhas, Krishna P.; Cheriyan, Vino T.; Puliappadamba, Vineshkumar T.; Bava, Smitha V.; Unnithan, Rajesh G.; Vijayammal, Parvathy L.; Anto, Ruby John.

In: Journal of Cellular and Molecular Medicine, Vol. 14, No. 3, 01.03.2010, p. 636-646.

Research output: Contribution to journalArticle

Laladhas, Krishna P. ; Cheriyan, Vino T. ; Puliappadamba, Vineshkumar T. ; Bava, Smitha V. ; Unnithan, Rajesh G. ; Vijayammal, Parvathy L. ; Anto, Ruby John. / A novel protein fraction from Sesbania grandiflora shows potential anticancer and chemopreventive efficacy, in vitro and in vivo. In: Journal of Cellular and Molecular Medicine. 2010 ; Vol. 14, No. 3. pp. 636-646.
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