A novel protein fraction from Sesbania grandiflora shows potential anticancer and chemopreventive efficacy, in vitro and in vivo

Krishna P. Laladhas, Vino T. Cheriyan, Vineshkumar T. Puliappadamba, Smitha V. Bava, Rajesh G. Unnithan, Parvathy L. Vijayammal, Ruby John Anto

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

We report mechanism-based evidence for the anticancer efficacy of a protein fraction, SF2 (Sesbania fraction 2) isolated from the flower of the medicinal plant, Sesbania grandiflora (S. grandiflora). The fraction was evaluated in two murine ascites tumour cell lines and human cancer cell lines of different origin for its anticancer effect. SF2 inhibited cell proliferation and induced apoptosis as demonstrated by DNA fragmentation and externalization of phosphatidyl serine in Daltons lymphoma ascites (DLA) and colon cancer cells (SW-480). Sensitivity to SF2 in these cells was associated with activation of caspases 3, 8 and 9, poly (ADP-ribose) polymerase cleavage and cytochrome C release which attests apoptosis induced cell death. Mechanistically, SF2 down-regulated phorbol myristate acetate (PMA) induced NF-κB, a transcription factor which controls the expression of genes encoding proteins involved in cell regulation and growth control. Additionally, SF2 also down-regulated anti-apoptotic factors such as Bcl-2, p-Akt and cyclooxygenase-2 induced by the tumour promoter PMA suggestive of a possible explanation for its anticancer effect. In vivo studies using ascites and solid tumour models strongly support in vitro findings as SF2 administration increased the life span and decreased the tumour volume in mice bearing tumour. In vivo toxicological evaluation revealed the pharmacological safety of SF2 and may serve as a potential anticancer drug candidate.

Original languageEnglish (US)
Pages (from-to)636-646
Number of pages11
JournalJournal of Cellular and Molecular Medicine
Volume14
Issue number3
DOIs
StatePublished - Mar 1 2010

Keywords

  • Anticancer
  • Apoptosis
  • Chemoprevention
  • Sesbania grandiflora

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology

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