A novel recombinant vaccine which protects mice against ricin intoxication

Joan E. Smallshaw, Ana Firan, John R. Fulmer, Stephen L. Ruback, Victor Ghetie, Ellen S. Vitetta

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

Ricin toxin (RT) is a plant-derived toxin of extraordinary toxicity; a single molecule successfully internalized into the cytoplasm of a cell is lethal for that cell. An estimated dose of 1-10μg/kg is lethal to humans, making aerosolized ricin a potential agent for bioterrorism. Vaccination against ricin using either denatured toxin or its modified A chain subunit (RTA) has been successful in experimental animals but both vaccines have potential toxicities. Recombinant (r) RTA has not been evaluated as a vaccine. However, the advantage of such a vaccine is that these potential toxicities can be deleted by appropriate mutations. In this study we have generated three mutants and shown that two lack toxicity as compared to the wild type rRTA. These mutants induce protective humoral immune responses in mice. One or both should be considered for use in humans.

Original languageEnglish (US)
Pages (from-to)3422-3427
Number of pages6
JournalVaccine
Volume20
Issue number27-28
DOIs
StatePublished - Sep 10 2002

Keywords

  • Bioterrorism
  • Mutation
  • Ricin

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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  • Cite this

    Smallshaw, J. E., Firan, A., Fulmer, J. R., Ruback, S. L., Ghetie, V., & Vitetta, E. S. (2002). A novel recombinant vaccine which protects mice against ricin intoxication. Vaccine, 20(27-28), 3422-3427. https://doi.org/10.1016/S0264-410X(02)00312-2