A novel view of albumin-supported sperm capacitation: Role of Lipid Transfer Protein-I

S. E. Ravnik, J. J. Albers, C. H. Muller

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Objective: To determine if one mechanism of albumin-mediated support of human sperm capacitation is lipid (cholesterol) transfer activity and contamination of albumin with Lipid Transfer Protein-I (LTP-I). Design and Main Outcome Measures: Measure lipid transfer activity in various bovine and human albumin preparations; relate this activity to albumin-supported capacitation (measured by zona-free hamster oocyte sperm penetration assay) and acrosome reactions; and attempt to detect LTP-I in active albumins. Remove LTP-I from albumin which supports capacitation and reassess this support. Reconstitute capacitation support by addition of purified LTP-I. Setting and Subjects: Healthy sperm donors with normal semen analyses were recruited by the Reproductive Biology-Andrology Laboratory in a university medical center. Results: Albumin preparations that effectively support capacitation have high levels of lipid transfer activity and of LTP-I, a protein responsible for lipid transfer activity. Preparations with lower levels of capacitation support have less lipid transfer activity. Removal of LTP-I from supportive albumin significantly reduces the capacitation support, and this is restored by purified LTP-I. Progesterone concentrations in these preparations are negligible. Conclusions: The variable abilities of albumin preparations to support in vitro sperm capacitation are largely dependent on the presence of contaminating LTP-I. The cholesterol transfer activity of this protein, which is present in human serum and follicular fluid, may be one mechanism in the process of capacitation.

Original languageEnglish (US)
Pages (from-to)629-638
Number of pages10
JournalFertility and Sterility
Volume59
Issue number3
StatePublished - 1993

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Sperm Capacitation
Albumins
Lipids
Cholesterol
Andrology
Sperm-Ovum Interactions
lipid transfer protein
Acrosome Reaction
Follicular Fluid
Semen Analysis
Herpes Zoster
Cricetinae
Progesterone
Spermatozoa
Healthy Volunteers
Outcome Assessment (Health Care)
Tissue Donors

Keywords

  • acrosome reaction
  • albumin
  • cholesterol
  • Human sperm capacitation
  • lipid transfer protein
  • serum
  • zona-free hamster oocyte sperm penetration assay

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

A novel view of albumin-supported sperm capacitation : Role of Lipid Transfer Protein-I. / Ravnik, S. E.; Albers, J. J.; Muller, C. H.

In: Fertility and Sterility, Vol. 59, No. 3, 1993, p. 629-638.

Research output: Contribution to journalArticle

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N2 - Objective: To determine if one mechanism of albumin-mediated support of human sperm capacitation is lipid (cholesterol) transfer activity and contamination of albumin with Lipid Transfer Protein-I (LTP-I). Design and Main Outcome Measures: Measure lipid transfer activity in various bovine and human albumin preparations; relate this activity to albumin-supported capacitation (measured by zona-free hamster oocyte sperm penetration assay) and acrosome reactions; and attempt to detect LTP-I in active albumins. Remove LTP-I from albumin which supports capacitation and reassess this support. Reconstitute capacitation support by addition of purified LTP-I. Setting and Subjects: Healthy sperm donors with normal semen analyses were recruited by the Reproductive Biology-Andrology Laboratory in a university medical center. Results: Albumin preparations that effectively support capacitation have high levels of lipid transfer activity and of LTP-I, a protein responsible for lipid transfer activity. Preparations with lower levels of capacitation support have less lipid transfer activity. Removal of LTP-I from supportive albumin significantly reduces the capacitation support, and this is restored by purified LTP-I. Progesterone concentrations in these preparations are negligible. Conclusions: The variable abilities of albumin preparations to support in vitro sperm capacitation are largely dependent on the presence of contaminating LTP-I. The cholesterol transfer activity of this protein, which is present in human serum and follicular fluid, may be one mechanism in the process of capacitation.

AB - Objective: To determine if one mechanism of albumin-mediated support of human sperm capacitation is lipid (cholesterol) transfer activity and contamination of albumin with Lipid Transfer Protein-I (LTP-I). Design and Main Outcome Measures: Measure lipid transfer activity in various bovine and human albumin preparations; relate this activity to albumin-supported capacitation (measured by zona-free hamster oocyte sperm penetration assay) and acrosome reactions; and attempt to detect LTP-I in active albumins. Remove LTP-I from albumin which supports capacitation and reassess this support. Reconstitute capacitation support by addition of purified LTP-I. Setting and Subjects: Healthy sperm donors with normal semen analyses were recruited by the Reproductive Biology-Andrology Laboratory in a university medical center. Results: Albumin preparations that effectively support capacitation have high levels of lipid transfer activity and of LTP-I, a protein responsible for lipid transfer activity. Preparations with lower levels of capacitation support have less lipid transfer activity. Removal of LTP-I from supportive albumin significantly reduces the capacitation support, and this is restored by purified LTP-I. Progesterone concentrations in these preparations are negligible. Conclusions: The variable abilities of albumin preparations to support in vitro sperm capacitation are largely dependent on the presence of contaminating LTP-I. The cholesterol transfer activity of this protein, which is present in human serum and follicular fluid, may be one mechanism in the process of capacitation.

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