A novel way to statistically analyze morphologic changes in Dmp1-null osteocytes

Y. Ren, S. Lin, Y. Jing, P. C. Dechow, Jian Q. Feng

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Recent studies have revealed multiple roles of osteocytes in bone metabolism. However, detailed analyses of the embedded osteocytes in bone structure are still limited because of the high mineral content around these cells. In this study, we developed an innovative technique, the "FITC-Imaris technique", which combines FITC ([2, 5]-Fluorescein isothiocyanate), confocal microscopy and Imaris software. With this method, we could not only visualize the 3-D morphology of embedded osteocytes, but more importantly, we were able to statistically quantitate the osteocyte structure in the cell surface area, total cell volume, and dendrite numbers. Furthermore, we made a side-by-side comparison of the new method with the acid-etched SEM imaging technique, a common imaging method for studies of osteocyte morphology with a much smaller cell depth (< 3 mm). Finally, we used the FITC-Imaris technique to show both the morphological and statistical differences in the osteocyte structure between the Dmp1-null mice (the osteomalacia model) and their age-matched control littermates. We expect that this newly developed technique will become a powerful tool to disclose more roles that osteocytes play in bone health and diseases.

Original languageEnglish (US)
Pages (from-to)129-133
Number of pages5
JournalConnective Tissue Research
Volume55
Issue numberSUPPL. 1
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Osteocytes
Fluorescein-5-isothiocyanate
Bone
Imaging techniques
Confocal microscopy
Metabolism
Minerals
Health
Scanning electron microscopy
Acids
Bone and Bones
Osteomalacia
Bone Diseases
Dendrites
Cell Size
Confocal Microscopy
Software

Keywords

  • DMP1
  • FITC
  • Imaris
  • Osteocyte quantification

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology

Cite this

A novel way to statistically analyze morphologic changes in Dmp1-null osteocytes. / Ren, Y.; Lin, S.; Jing, Y.; Dechow, P. C.; Feng, Jian Q.

In: Connective Tissue Research, Vol. 55, No. SUPPL. 1, 01.01.2014, p. 129-133.

Research output: Contribution to journalArticle

Ren, Y. ; Lin, S. ; Jing, Y. ; Dechow, P. C. ; Feng, Jian Q. / A novel way to statistically analyze morphologic changes in Dmp1-null osteocytes. In: Connective Tissue Research. 2014 ; Vol. 55, No. SUPPL. 1. pp. 129-133.
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