A novel zinc finger protein mRNA in human umbilical vein endothelial cells is profoundly induced by tumor necrosis factor alpha.

C. Mataki, T. Murakami, M. Umetani, Y. Wada, M. Ishii, S. Tsutsumi, H. Aburatani, T. Hamakubo, T. Kodama

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Zinc finger proteins are known to mediate various transcriptional control mechanisms and other cellular functions in human cells. Tumor necrosis factor alpha (TNFalpha) induces a variety of genes in human endothelial cells including A20, an antiapoptotic zinc finger protein. In order to identify other zinc finger protein genes induced by TNFalpha, we studied the gene expression profile of human umbilical vein endothelial cells (HUVECs) stimulated by TNFalpha by means of oligonucleotide microarrays. Among the 155 genes encoding zinc finger motif, the level of EST M88357 mRNA encoding a novel designated EZFIT (endothelial zinc finger protein induced by TNFalpha) was induced most profoundly (>19 fold). The EZFIT gene is located on the chromosome 19q13.4. Isolation of the full length cDNA coding sequence by PCR using primers architected from the genomic sequence revealed that EZFIT has 490 amino acids which contain 13 C2H2 zinc finger motifs. Among 24 human organs and cell types studied, EZFIT mRNA was found to be most highly expressed in the placenta followed by the brain, testis, pancreas, heart, small intestine, muscle, uterus, prostate and peripheral blood leukocytes. EZFIT mRNA was not detected in the liver, lung, colon, stomach, the salivary gland or the thyroid gland.

Original languageEnglish (US)
Pages (from-to)97-103
Number of pages7
JournalJournal of Atherosclerosis and Thrombosis
Volume7
Issue number2
StatePublished - 2000

Fingerprint

Zinc Fingers
Endothelial cells
Human Umbilical Vein Endothelial Cells
Zinc
Tumor Necrosis Factor-alpha
Messenger RNA
Proteins
Endothelial Cells
Genes
Expressed Sequence Tags
Gene encoding
Salivary Glands
Oligonucleotide Array Sequence Analysis
Transcriptome
Microarrays
Chromosomes
Placenta
Small Intestine
Uterus
Gene expression

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Internal Medicine
  • Biochemistry, medical

Cite this

Mataki, C., Murakami, T., Umetani, M., Wada, Y., Ishii, M., Tsutsumi, S., ... Kodama, T. (2000). A novel zinc finger protein mRNA in human umbilical vein endothelial cells is profoundly induced by tumor necrosis factor alpha. Journal of Atherosclerosis and Thrombosis, 7(2), 97-103.

A novel zinc finger protein mRNA in human umbilical vein endothelial cells is profoundly induced by tumor necrosis factor alpha. / Mataki, C.; Murakami, T.; Umetani, M.; Wada, Y.; Ishii, M.; Tsutsumi, S.; Aburatani, H.; Hamakubo, T.; Kodama, T.

In: Journal of Atherosclerosis and Thrombosis, Vol. 7, No. 2, 2000, p. 97-103.

Research output: Contribution to journalArticle

Mataki, C, Murakami, T, Umetani, M, Wada, Y, Ishii, M, Tsutsumi, S, Aburatani, H, Hamakubo, T & Kodama, T 2000, 'A novel zinc finger protein mRNA in human umbilical vein endothelial cells is profoundly induced by tumor necrosis factor alpha.', Journal of Atherosclerosis and Thrombosis, vol. 7, no. 2, pp. 97-103.
Mataki, C. ; Murakami, T. ; Umetani, M. ; Wada, Y. ; Ishii, M. ; Tsutsumi, S. ; Aburatani, H. ; Hamakubo, T. ; Kodama, T. / A novel zinc finger protein mRNA in human umbilical vein endothelial cells is profoundly induced by tumor necrosis factor alpha. In: Journal of Atherosclerosis and Thrombosis. 2000 ; Vol. 7, No. 2. pp. 97-103.
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