A p53 enhancer region regulates target genes through chromatin conformations in cis and in trans

Nichole Link, Paula Kurtz, Melissa O'Neal, Gianella Garcia-Hughes, John M. Abrams

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We examined how a p53 enhancer transmits regulatory information in vivo. Using genetic ablation together with digital chromosome conformation capture and fluorescent in situ hybridization, we found that a Drosophila p53 enhancer region (referred to as the p53 response element [p53RE]) physically contacts targets in cis and across the centromere to control stress-responsive transcription at these sites. Furthermore, when placed at ectopic genomic positions, fragments spanning this element re-established chromatin contacts and partially restored target gene regulation to mutants lacking the native p53RE. Therefore, a defined p53 enhancer region is sufficient for longrange chromatin interactions that enable multigenic regulation.

Original languageEnglish (US)
Pages (from-to)2433-2438
Number of pages6
JournalGenes and Development
Volume27
Issue number22
DOIs
StatePublished - Nov 15 2013

Fingerprint

Response Elements
Chromatin
Centromere
Fluorescence In Situ Hybridization
Genes
Drosophila
Chromosomes

Keywords

  • Apoptosis
  • Chromosome conformation capture
  • Genome architecture
  • Long-distance gene regulation
  • P53

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

A p53 enhancer region regulates target genes through chromatin conformations in cis and in trans. / Link, Nichole; Kurtz, Paula; O'Neal, Melissa; Garcia-Hughes, Gianella; Abrams, John M.

In: Genes and Development, Vol. 27, No. 22, 15.11.2013, p. 2433-2438.

Research output: Contribution to journalArticle

Link, Nichole ; Kurtz, Paula ; O'Neal, Melissa ; Garcia-Hughes, Gianella ; Abrams, John M. / A p53 enhancer region regulates target genes through chromatin conformations in cis and in trans. In: Genes and Development. 2013 ; Vol. 27, No. 22. pp. 2433-2438.
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