A Periodontal pathogen Porphyromonas gingivalis deteriorates Isoproterenol-Induced myocardial remodeling in mice

Hiroki Sato, Jun Ichi Suzuki, Norio Aoyama, Ryo Watanabe, Makoto Kaneko, Yuka Shiheido, Asuka Yoshida, Kouji Wakayama, Hidetoshi Kumagai, Yuichi Ikeda, Hiroshi Akazawa, Issei Komuro, Mitsuaki Isobe, Yuichi Izumi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Heart failure is a serious disease induced by several conditions, including hypertrophic cardiomyopathy. Although many reports suggest that there is an association between periodontal disease and cardiovascular disease, the mechanisms have yet to be elucidated. The purpose of this study was to clarify the relationship between periodontal disease and heart disease, especially in cardiac hypertrophy. We used C57BL/6J mice and implanted two types of subcutaneous chambers. First, we subcutaneously implanted a coil-shaped chamber into the back of a mouse. Porphyromonas gingivalis (P.g.), a major periodontal pathogen, was injected into the chamber. Then, an osmotic pump was implanted to infuse isoproterenol. Four weeks after the ISO infusion, we performed echocardiography and harvested the heart and blood. We measured the serum level of anti-P.g.-IgG using ELISA. The mRNA levels of several factors were measured using PCR. We found stronger cardiomyocyte hypertrophy in the ISO(+)/P.g.(+) mice compared with the ISO(+)/P.g.(-) mice. The total square of randomly selected cardiomyocytes was 23% larger in the ISO(+)/P.g.(+) mice than in the ISO(+)/P.g.(-) mice. We detected a higher level of mRNA expression in Toll-like receptor 2 and NADPH oxidase 4 in the ISO(+)/P.g.(-) mice compared with the control group. We revealed that a periodontal pathogen affected ISO-induced cardiac hypertrophy via oxidative stress.

Original languageEnglish (US)
Pages (from-to)35-40
Number of pages6
JournalHypertension Research
Volume40
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

Porphyromonas gingivalis
Isoproterenol
Cardiomegaly
Periodontal Diseases
Cardiac Myocytes
Toll-Like Receptor 2
Messenger RNA
NADPH Oxidase
Hypertrophic Cardiomyopathy
Inbred C57BL Mouse
Hypertrophy
Echocardiography
Heart Diseases
Oxidative Stress
Cardiovascular Diseases
Heart Failure
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Polymerase Chain Reaction
Control Groups

Keywords

  • Cardiac hypertrophy
  • Isoproterenol
  • Periodontal disease
  • Porphyromonas gingivalis
  • Toll-like receptor

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Sato, H., Suzuki, J. I., Aoyama, N., Watanabe, R., Kaneko, M., Shiheido, Y., ... Izumi, Y. (2017). A Periodontal pathogen Porphyromonas gingivalis deteriorates Isoproterenol-Induced myocardial remodeling in mice. Hypertension Research, 40(1), 35-40. https://doi.org/10.1038/hr.2016.114

A Periodontal pathogen Porphyromonas gingivalis deteriorates Isoproterenol-Induced myocardial remodeling in mice. / Sato, Hiroki; Suzuki, Jun Ichi; Aoyama, Norio; Watanabe, Ryo; Kaneko, Makoto; Shiheido, Yuka; Yoshida, Asuka; Wakayama, Kouji; Kumagai, Hidetoshi; Ikeda, Yuichi; Akazawa, Hiroshi; Komuro, Issei; Isobe, Mitsuaki; Izumi, Yuichi.

In: Hypertension Research, Vol. 40, No. 1, 01.01.2017, p. 35-40.

Research output: Contribution to journalArticle

Sato, H, Suzuki, JI, Aoyama, N, Watanabe, R, Kaneko, M, Shiheido, Y, Yoshida, A, Wakayama, K, Kumagai, H, Ikeda, Y, Akazawa, H, Komuro, I, Isobe, M & Izumi, Y 2017, 'A Periodontal pathogen Porphyromonas gingivalis deteriorates Isoproterenol-Induced myocardial remodeling in mice', Hypertension Research, vol. 40, no. 1, pp. 35-40. https://doi.org/10.1038/hr.2016.114
Sato, Hiroki ; Suzuki, Jun Ichi ; Aoyama, Norio ; Watanabe, Ryo ; Kaneko, Makoto ; Shiheido, Yuka ; Yoshida, Asuka ; Wakayama, Kouji ; Kumagai, Hidetoshi ; Ikeda, Yuichi ; Akazawa, Hiroshi ; Komuro, Issei ; Isobe, Mitsuaki ; Izumi, Yuichi. / A Periodontal pathogen Porphyromonas gingivalis deteriorates Isoproterenol-Induced myocardial remodeling in mice. In: Hypertension Research. 2017 ; Vol. 40, No. 1. pp. 35-40.
@article{28c9e25e9d8c48f680156caabb8a37ae,
title = "A Periodontal pathogen Porphyromonas gingivalis deteriorates Isoproterenol-Induced myocardial remodeling in mice",
abstract = "Heart failure is a serious disease induced by several conditions, including hypertrophic cardiomyopathy. Although many reports suggest that there is an association between periodontal disease and cardiovascular disease, the mechanisms have yet to be elucidated. The purpose of this study was to clarify the relationship between periodontal disease and heart disease, especially in cardiac hypertrophy. We used C57BL/6J mice and implanted two types of subcutaneous chambers. First, we subcutaneously implanted a coil-shaped chamber into the back of a mouse. Porphyromonas gingivalis (P.g.), a major periodontal pathogen, was injected into the chamber. Then, an osmotic pump was implanted to infuse isoproterenol. Four weeks after the ISO infusion, we performed echocardiography and harvested the heart and blood. We measured the serum level of anti-P.g.-IgG using ELISA. The mRNA levels of several factors were measured using PCR. We found stronger cardiomyocyte hypertrophy in the ISO(+)/P.g.(+) mice compared with the ISO(+)/P.g.(-) mice. The total square of randomly selected cardiomyocytes was 23{\%} larger in the ISO(+)/P.g.(+) mice than in the ISO(+)/P.g.(-) mice. We detected a higher level of mRNA expression in Toll-like receptor 2 and NADPH oxidase 4 in the ISO(+)/P.g.(-) mice compared with the control group. We revealed that a periodontal pathogen affected ISO-induced cardiac hypertrophy via oxidative stress.",
keywords = "Cardiac hypertrophy, Isoproterenol, Periodontal disease, Porphyromonas gingivalis, Toll-like receptor",
author = "Hiroki Sato and Suzuki, {Jun Ichi} and Norio Aoyama and Ryo Watanabe and Makoto Kaneko and Yuka Shiheido and Asuka Yoshida and Kouji Wakayama and Hidetoshi Kumagai and Yuichi Ikeda and Hiroshi Akazawa and Issei Komuro and Mitsuaki Isobe and Yuichi Izumi",
year = "2017",
month = "1",
day = "1",
doi = "10.1038/hr.2016.114",
language = "English (US)",
volume = "40",
pages = "35--40",
journal = "Hypertension Research",
issn = "0916-9636",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - A Periodontal pathogen Porphyromonas gingivalis deteriorates Isoproterenol-Induced myocardial remodeling in mice

AU - Sato, Hiroki

AU - Suzuki, Jun Ichi

AU - Aoyama, Norio

AU - Watanabe, Ryo

AU - Kaneko, Makoto

AU - Shiheido, Yuka

AU - Yoshida, Asuka

AU - Wakayama, Kouji

AU - Kumagai, Hidetoshi

AU - Ikeda, Yuichi

AU - Akazawa, Hiroshi

AU - Komuro, Issei

AU - Isobe, Mitsuaki

AU - Izumi, Yuichi

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Heart failure is a serious disease induced by several conditions, including hypertrophic cardiomyopathy. Although many reports suggest that there is an association between periodontal disease and cardiovascular disease, the mechanisms have yet to be elucidated. The purpose of this study was to clarify the relationship between periodontal disease and heart disease, especially in cardiac hypertrophy. We used C57BL/6J mice and implanted two types of subcutaneous chambers. First, we subcutaneously implanted a coil-shaped chamber into the back of a mouse. Porphyromonas gingivalis (P.g.), a major periodontal pathogen, was injected into the chamber. Then, an osmotic pump was implanted to infuse isoproterenol. Four weeks after the ISO infusion, we performed echocardiography and harvested the heart and blood. We measured the serum level of anti-P.g.-IgG using ELISA. The mRNA levels of several factors were measured using PCR. We found stronger cardiomyocyte hypertrophy in the ISO(+)/P.g.(+) mice compared with the ISO(+)/P.g.(-) mice. The total square of randomly selected cardiomyocytes was 23% larger in the ISO(+)/P.g.(+) mice than in the ISO(+)/P.g.(-) mice. We detected a higher level of mRNA expression in Toll-like receptor 2 and NADPH oxidase 4 in the ISO(+)/P.g.(-) mice compared with the control group. We revealed that a periodontal pathogen affected ISO-induced cardiac hypertrophy via oxidative stress.

AB - Heart failure is a serious disease induced by several conditions, including hypertrophic cardiomyopathy. Although many reports suggest that there is an association between periodontal disease and cardiovascular disease, the mechanisms have yet to be elucidated. The purpose of this study was to clarify the relationship between periodontal disease and heart disease, especially in cardiac hypertrophy. We used C57BL/6J mice and implanted two types of subcutaneous chambers. First, we subcutaneously implanted a coil-shaped chamber into the back of a mouse. Porphyromonas gingivalis (P.g.), a major periodontal pathogen, was injected into the chamber. Then, an osmotic pump was implanted to infuse isoproterenol. Four weeks after the ISO infusion, we performed echocardiography and harvested the heart and blood. We measured the serum level of anti-P.g.-IgG using ELISA. The mRNA levels of several factors were measured using PCR. We found stronger cardiomyocyte hypertrophy in the ISO(+)/P.g.(+) mice compared with the ISO(+)/P.g.(-) mice. The total square of randomly selected cardiomyocytes was 23% larger in the ISO(+)/P.g.(+) mice than in the ISO(+)/P.g.(-) mice. We detected a higher level of mRNA expression in Toll-like receptor 2 and NADPH oxidase 4 in the ISO(+)/P.g.(-) mice compared with the control group. We revealed that a periodontal pathogen affected ISO-induced cardiac hypertrophy via oxidative stress.

KW - Cardiac hypertrophy

KW - Isoproterenol

KW - Periodontal disease

KW - Porphyromonas gingivalis

KW - Toll-like receptor

UR - http://www.scopus.com/inward/record.url?scp=85008385952&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85008385952&partnerID=8YFLogxK

U2 - 10.1038/hr.2016.114

DO - 10.1038/hr.2016.114

M3 - Article

C2 - 27604343

AN - SCOPUS:85008385952

VL - 40

SP - 35

EP - 40

JO - Hypertension Research

JF - Hypertension Research

SN - 0916-9636

IS - 1

ER -