TY - JOUR
T1 - A Plant Pathogen Type III Effector Protein Subverts Translational Regulation to Boost Host Polyamine Levels
AU - Wu, Dousheng
AU - von Roepenack-Lahaye, Edda
AU - Buntru, Matthias
AU - de Lange, Orlando
AU - Schandry, Niklas
AU - Pérez-Quintero, Alvaro L.
AU - Weinberg, Zasha
AU - Lowe-Power, Tiffany M.
AU - Szurek, Boris
AU - Michael, Anthony J.
AU - Allen, Caitilyn
AU - Schillberg, Stefan
AU - Lahaye, Thomas
N1 - Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/11/13
Y1 - 2019/11/13
N2 - Pathogenic bacteria inject effector proteins into host cells to manipulate cellular processes and facilitate the infection. Transcription-activator-like effectors (TALEs), an effector class in plant pathogenic bacteria, transcriptionally activate host genes to promote disease. We identify arginine decarboxylase (ADC) genes as the host targets of Brg11, a TALE-like effector from the plant pathogen Ralstonia solanacearum. Brg11 targets a 17-bp sequence that was found to be part of a conserved 50-bp motif, termed the ADC-box, upstream of ADC genes involved in polyamine biosynthesis. The transcribed ADC-box attenuates translation from native ADC mRNAs; however, Brg11 induces truncated ADC mRNAs lacking the ADC-box, thus bypassing this translational control. As a result, Brg11 induces elevated polyamine levels that trigger a defense reaction and likely inhibits bacterial niche competitors but not R. solanacearum. Our findings suggest that Brg11 may give R. solanacearum a competitive advantage and uncover a role for bacterial effectors in regulating ternary microbe-host-microbe interactions.
AB - Pathogenic bacteria inject effector proteins into host cells to manipulate cellular processes and facilitate the infection. Transcription-activator-like effectors (TALEs), an effector class in plant pathogenic bacteria, transcriptionally activate host genes to promote disease. We identify arginine decarboxylase (ADC) genes as the host targets of Brg11, a TALE-like effector from the plant pathogen Ralstonia solanacearum. Brg11 targets a 17-bp sequence that was found to be part of a conserved 50-bp motif, termed the ADC-box, upstream of ADC genes involved in polyamine biosynthesis. The transcribed ADC-box attenuates translation from native ADC mRNAs; however, Brg11 induces truncated ADC mRNAs lacking the ADC-box, thus bypassing this translational control. As a result, Brg11 induces elevated polyamine levels that trigger a defense reaction and likely inhibits bacterial niche competitors but not R. solanacearum. Our findings suggest that Brg11 may give R. solanacearum a competitive advantage and uncover a role for bacterial effectors in regulating ternary microbe-host-microbe interactions.
KW - Ralstonia solanacearum
KW - arginine decarboxylase (ADC)
KW - microbiota
KW - polyamines
KW - putrescine
KW - rhizosphere
KW - translational regulation
KW - type III effectors
KW - upstream open reading frame (uORF)
UR - http://www.scopus.com/inward/record.url?scp=85074421624&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85074421624&partnerID=8YFLogxK
U2 - 10.1016/j.chom.2019.09.014
DO - 10.1016/j.chom.2019.09.014
M3 - Article
C2 - 31628081
AN - SCOPUS:85074421624
SN - 1931-3128
VL - 26
SP - 638-649.e5
JO - Cell Host and Microbe
JF - Cell Host and Microbe
IS - 5
ER -