A Plant Pathogen Type III Effector Protein Subverts Translational Regulation to Boost Host Polyamine Levels

Dousheng Wu, Edda von Roepenack-Lahaye, Matthias Buntru, Orlando de Lange, Niklas Schandry, Alvaro L. Pérez-Quintero, Zasha Weinberg, Tiffany M. Lowe-Power, Boris Szurek, Anthony J. Michael, Caitilyn Allen, Stefan Schillberg, Thomas Lahaye

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Pathogenic bacteria inject effector proteins into host cells to manipulate cellular processes and facilitate the infection. Transcription-activator-like effectors (TALEs), an effector class in plant pathogenic bacteria, transcriptionally activate host genes to promote disease. We identify arginine decarboxylase (ADC) genes as the host targets of Brg11, a TALE-like effector from the plant pathogen Ralstonia solanacearum. Brg11 targets a 17-bp sequence that was found to be part of a conserved 50-bp motif, termed the ADC-box, upstream of ADC genes involved in polyamine biosynthesis. The transcribed ADC-box attenuates translation from native ADC mRNAs; however, Brg11 induces truncated ADC mRNAs lacking the ADC-box, thus bypassing this translational control. As a result, Brg11 induces elevated polyamine levels that trigger a defense reaction and likely inhibits bacterial niche competitors but not R. solanacearum. Our findings suggest that Brg11 may give R. solanacearum a competitive advantage and uncover a role for bacterial effectors in regulating ternary microbe-host-microbe interactions.

Original languageEnglish (US)
Pages (from-to)638-649.e5
JournalCell Host and Microbe
Volume26
Issue number5
DOIs
StatePublished - Nov 13 2019

Fingerprint

Polyamines
Ralstonia solanacearum
Proteins
Genes
Bacteria
Messenger RNA
arginine decarboxylase
Infection

Keywords

  • arginine decarboxylase (ADC)
  • microbiota
  • polyamines
  • putrescine
  • Ralstonia solanacearum
  • rhizosphere
  • translational regulation
  • type III effectors
  • upstream open reading frame (uORF)

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

Cite this

Wu, D., von Roepenack-Lahaye, E., Buntru, M., de Lange, O., Schandry, N., Pérez-Quintero, A. L., ... Lahaye, T. (2019). A Plant Pathogen Type III Effector Protein Subverts Translational Regulation to Boost Host Polyamine Levels. Cell Host and Microbe, 26(5), 638-649.e5. https://doi.org/10.1016/j.chom.2019.09.014

A Plant Pathogen Type III Effector Protein Subverts Translational Regulation to Boost Host Polyamine Levels. / Wu, Dousheng; von Roepenack-Lahaye, Edda; Buntru, Matthias; de Lange, Orlando; Schandry, Niklas; Pérez-Quintero, Alvaro L.; Weinberg, Zasha; Lowe-Power, Tiffany M.; Szurek, Boris; Michael, Anthony J.; Allen, Caitilyn; Schillberg, Stefan; Lahaye, Thomas.

In: Cell Host and Microbe, Vol. 26, No. 5, 13.11.2019, p. 638-649.e5.

Research output: Contribution to journalArticle

Wu, D, von Roepenack-Lahaye, E, Buntru, M, de Lange, O, Schandry, N, Pérez-Quintero, AL, Weinberg, Z, Lowe-Power, TM, Szurek, B, Michael, AJ, Allen, C, Schillberg, S & Lahaye, T 2019, 'A Plant Pathogen Type III Effector Protein Subverts Translational Regulation to Boost Host Polyamine Levels', Cell Host and Microbe, vol. 26, no. 5, pp. 638-649.e5. https://doi.org/10.1016/j.chom.2019.09.014
Wu D, von Roepenack-Lahaye E, Buntru M, de Lange O, Schandry N, Pérez-Quintero AL et al. A Plant Pathogen Type III Effector Protein Subverts Translational Regulation to Boost Host Polyamine Levels. Cell Host and Microbe. 2019 Nov 13;26(5):638-649.e5. https://doi.org/10.1016/j.chom.2019.09.014
Wu, Dousheng ; von Roepenack-Lahaye, Edda ; Buntru, Matthias ; de Lange, Orlando ; Schandry, Niklas ; Pérez-Quintero, Alvaro L. ; Weinberg, Zasha ; Lowe-Power, Tiffany M. ; Szurek, Boris ; Michael, Anthony J. ; Allen, Caitilyn ; Schillberg, Stefan ; Lahaye, Thomas. / A Plant Pathogen Type III Effector Protein Subverts Translational Regulation to Boost Host Polyamine Levels. In: Cell Host and Microbe. 2019 ; Vol. 26, No. 5. pp. 638-649.e5.
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