A platform for high-throughput identification and characterization of intact proteins from Methanococcus jannaschii

Andrew J. Forbes, Jeffrey R. Johnson, Steven M. Patrie, Neil L. Kelleher

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The development of general methods for identifying and characterizing intact proteins in high throughput manner, using native proteins from Methanococcus jannaschii, was discussed. A 2-D separation to sufficiently fractionate the soluble portion of the proteome, followed by automated fourier transform mass spectrometry (FTMS) and MS/MS for the proteins in these samples, with a modified THRASH algorithm were used to analyze the data. The soluble portion of the lysate was separated via preparative denatured polyacrylamide gel electrophoresis. The protein precipitation had demonstrated high recovery, and the resolution of the HPLC traces indicated that the acid-labile surfactant (ALS) was both removed or degraded by the precipitation steps.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages239-240
Number of pages2
StatePublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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    Forbes, A. J., Johnson, J. R., Patrie, S. M., & Kelleher, N. L. (2002). A platform for high-throughput identification and characterization of intact proteins from Methanococcus jannaschii. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 239-240)