TY - JOUR
T1 - A point mutation in Gα(o) and Gα(i1) blocks interaction with regulator of G protein signaling proteins
AU - Lan, Keng Li
AU - Sarvazyan, Noune A.
AU - Taussig, Ronald
AU - Mackenzie, Robert G.
AU - DiBello, Paul R.
AU - Dohlman, Henrik G.
AU - Neubig, Richard R.
PY - 1998/5/22
Y1 - 1998/5/22
N2 - Regulator of G protein-signaling (RGS) proteins accelerate GTP hydrolysis by Gα subunits and are thought to be responsible for rapid deactivation of enzymes and ion channels controlled by G proteins. We wanted to identify and characterize G(i)-family a subunits that were insensitive to RGS action. Based on a glycine to serine mutation in the yeast Gα subunit Gpa1(sst) that prevents deactivation by Sst2 (DiBello, P. R., Garrison, T. R., Apanovitch, D. M., Hoffman, G., Shuey, D. J., Mason, K., Cockett, M. I., and Dohlman, H. G. (1998) J. Biol. Chem. 273, 5780-5784), site-directed mutagenesis of α(o) and α(i1) was done. G184S α(o) and G183S α(i1) show kinetics of GDP release and GTP hydrolysis similar to wild type. In contrast, GTP hydrolysis by the G → S mutant proteins is not stimulated by RGS4 or by a truncated RGS7. Quantitative flow cytometry binding studies show IC50 values of 30 and 96 nM, respectively, for aluminum fluoride-activated wild type α(o) and all to compete with fluorescein isothiocyanate-α(o) binding to glutathione S-transferase-RGS4. The G → S mutant proteins showed a greater than 30-100-fold lower affinity for RGS4. Thus, we have defined the mechanism of a point mutation in α(o) and all that prevents RGS binding and GTPase activating activity. These mutant subunits should be useful in biochemical or expression studies to evaluate the role of endogenous RGS proteins in G(i) function.
AB - Regulator of G protein-signaling (RGS) proteins accelerate GTP hydrolysis by Gα subunits and are thought to be responsible for rapid deactivation of enzymes and ion channels controlled by G proteins. We wanted to identify and characterize G(i)-family a subunits that were insensitive to RGS action. Based on a glycine to serine mutation in the yeast Gα subunit Gpa1(sst) that prevents deactivation by Sst2 (DiBello, P. R., Garrison, T. R., Apanovitch, D. M., Hoffman, G., Shuey, D. J., Mason, K., Cockett, M. I., and Dohlman, H. G. (1998) J. Biol. Chem. 273, 5780-5784), site-directed mutagenesis of α(o) and α(i1) was done. G184S α(o) and G183S α(i1) show kinetics of GDP release and GTP hydrolysis similar to wild type. In contrast, GTP hydrolysis by the G → S mutant proteins is not stimulated by RGS4 or by a truncated RGS7. Quantitative flow cytometry binding studies show IC50 values of 30 and 96 nM, respectively, for aluminum fluoride-activated wild type α(o) and all to compete with fluorescein isothiocyanate-α(o) binding to glutathione S-transferase-RGS4. The G → S mutant proteins showed a greater than 30-100-fold lower affinity for RGS4. Thus, we have defined the mechanism of a point mutation in α(o) and all that prevents RGS binding and GTPase activating activity. These mutant subunits should be useful in biochemical or expression studies to evaluate the role of endogenous RGS proteins in G(i) function.
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U2 - 10.1074/jbc.273.21.12794
DO - 10.1074/jbc.273.21.12794
M3 - Article
C2 - 9582306
AN - SCOPUS:0032557248
SN - 0021-9258
VL - 273
SP - 12794
EP - 12797
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 21
ER -