A potent transactivation domain mimic with activity in living cells

Bo Liu, Prasanna G. Alluri, Peng Yu, Thomas Kodadek

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Transcriptional coactivator-binding peptoids were isolated from a large combinatorial library. One of these molecules is shown to function as a potent activation domain surrogate in mammalian cells. Up to a 900-fold increase in expression of a Gal4-responsive reporter gene is observed when a steroid conjugate of the peptoid is incubated with HeLa cells expressing a Gal4 DNA-binding domain (DBD)-glucocorticoid receptor ligand-binding domain (GRLBD) fusion protein.

Original languageEnglish (US)
Pages (from-to)8254-8255
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number23
DOIs
StatePublished - Jun 15 2005

Fingerprint

Peptoids
Transcriptional Activation
Cells
Glucocorticoid Receptors
Reporter Genes
HeLa Cells
DNA
Fusion reactions
Genes
Chemical activation
Steroids
Ligands
Proteins
Molecules

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

A potent transactivation domain mimic with activity in living cells. / Liu, Bo; Alluri, Prasanna G.; Yu, Peng; Kodadek, Thomas.

In: Journal of the American Chemical Society, Vol. 127, No. 23, 15.06.2005, p. 8254-8255.

Research output: Contribution to journalArticle

Liu, Bo ; Alluri, Prasanna G. ; Yu, Peng ; Kodadek, Thomas. / A potent transactivation domain mimic with activity in living cells. In: Journal of the American Chemical Society. 2005 ; Vol. 127, No. 23. pp. 8254-8255.
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