A rapid assay for circulating anti-glomerular basement membrane antibodies in Goodpasture syndrome

Ramesh Saxena, Bengt Isaksson, Per Bygren, Jörgen Wieslander

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

A rapid ELISA for the detection of circulating anti-glomerular basement membrane antibodies in Goodpasture syndrome is described. The specificity of the test was shown to be highly dependent on the antigens used. Using the purified Goodpasture antigen it was possible to shorten the incubation times to 10 min in a routine assay using alkaline phosphatase-labeled second antibodies and the total assay was complete in 30 min. 200 reference sera, 500 sera from patients with various types of glomerulonephritis and 32 sera from patients with Goodpasture syndrome were analyzed by this rapid assay. The assay was able to discriminate between Goodpasture syndrome and other forms of glomerulonephritis. Using enzyme amplification it was possible to further shorten the incubation times to 1 min and the total time of the assay to 6 min.

Original languageEnglish (US)
Pages (from-to)73-78
Number of pages6
JournalJournal of Immunological Methods
Volume118
Issue number1
DOIs
StatePublished - Mar 10 1989

Keywords

  • Anti-GBM antibody
  • ELISA
  • Enzyme amplification
  • Goodpasture antigen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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