A rapid change in virulence gene expression during the transition from the intestinal lumen into tissue promotes systemic dissemination of salmonella

Sebastian E. Winter, Maria G. Winter, Ivan Godinez, Hee Jeong Yang, Holger Rüssmann, Helene L. Andrews-Polymenis, Andreas J. Bäumler

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Bacterial pathogens causing systemic disease commonly evolve from organisms associated with localized infections but differ from their close relatives in their ability to overcome mucosal barriers by mechanisms that remain incompletely understood. Here we investigated whether acquisition of a regulatory gene, tviA, contributed to the ability of Salmonella enterica serotype Typhi to disseminate from the intestine to systemic sites of infection during typhoid fever. To study the consequences of acquiring a new regulator by horizontal gene transfer, tviA was introduced into the chromosome of S. enterica serotype Typhimurium, a closely related pathogen causing a localized gastrointestinal infection in immunocom-petent individuals. TviA repressed expression of flagellin, a pathogen associated molecular pattern (PAMP), when bacteria were grown at osmotic conditions encountered in tissue, but not at higher osmolarity present in the intestinal lumen. TviA-mediated flagellin repression enabled bacteria to evade sentinel functions of human model epithelia and resulted in increased bacterial dissemination to the spleen in a chicken model. Collectively, our data point to PAMP repression as a novel pathogenic mechanism to overcome the mucosal barrier through innate immune evasion.

Original languageEnglish (US)
Article numbere1001060
Pages (from-to)63-64
Number of pages2
JournalPLoS Pathogens
Volume6
Issue number8
DOIs
StatePublished - Aug 2010

Fingerprint

Salmonella
Virulence
Flagellin
Salmonella enterica
Regulator Genes
Gene Expression
Infection
Bacteria
Immune Evasion
Horizontal Gene Transfer
Typhoid Fever
Osmolar Concentration
Intestines
Chickens
Spleen
Epithelium
Chromosomes
Pathogen-Associated Molecular Pattern Molecules
Serogroup
cyhalothrin

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

A rapid change in virulence gene expression during the transition from the intestinal lumen into tissue promotes systemic dissemination of salmonella. / Winter, Sebastian E.; Winter, Maria G.; Godinez, Ivan; Yang, Hee Jeong; Rüssmann, Holger; Andrews-Polymenis, Helene L.; Bäumler, Andreas J.

In: PLoS Pathogens, Vol. 6, No. 8, e1001060, 08.2010, p. 63-64.

Research output: Contribution to journalArticle

Winter, Sebastian E. ; Winter, Maria G. ; Godinez, Ivan ; Yang, Hee Jeong ; Rüssmann, Holger ; Andrews-Polymenis, Helene L. ; Bäumler, Andreas J. / A rapid change in virulence gene expression during the transition from the intestinal lumen into tissue promotes systemic dissemination of salmonella. In: PLoS Pathogens. 2010 ; Vol. 6, No. 8. pp. 63-64.
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