A rapid, extensive, and transient transcriptional response to estrogen signaling in breast cancer cells

Nasun Hah, Charles G. Danko, Leighton Core, Joshua J. Waterfall, Adam Siepel, John T. Lis, W. Lee Kraus

Research output: Contribution to journalArticle

277 Citations (Scopus)

Abstract

We report the immediate effects of estrogen signaling on the transcriptome of breast cancer cells using global run-on and sequencing (GRO-seq). The data were analyzed using a new bioinformatic approach that allowed us to identify transcripts directly from the GRO-seq data. We found that estrogen signaling directly regulates a strikingly large fraction of the transcriptome in a rapid, robust, and unexpectedly transient manner. In addition to protein-coding genes, estrogen regulates the distribution and activity of all three RNA polymerases and virtually every class of noncoding RNA that has been described to date. We also identified a large number of previously undetected estrogen-regulated intergenic transcripts, many of which are found proximal to estrogen receptor binding sites. Collectively, our results provide the most comprehensive measurement of the primary and immediate estrogen effects to date and a resource for understanding rapid signal-dependent transcription in other systems.

Original languageEnglish (US)
Pages (from-to)622-634
Number of pages13
JournalCell
Volume145
Issue number4
DOIs
StatePublished - May 13 2011

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Transient analysis
Estrogens
Cells
Breast Neoplasms
Transcriptome
Untranslated RNA
DNA-Directed RNA Polymerases
Transcription
Bioinformatics
Computational Biology
Estrogen Receptors
Genes
Binding Sites
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A rapid, extensive, and transient transcriptional response to estrogen signaling in breast cancer cells. / Hah, Nasun; Danko, Charles G.; Core, Leighton; Waterfall, Joshua J.; Siepel, Adam; Lis, John T.; Kraus, W. Lee.

In: Cell, Vol. 145, No. 4, 13.05.2011, p. 622-634.

Research output: Contribution to journalArticle

Hah, Nasun ; Danko, Charles G. ; Core, Leighton ; Waterfall, Joshua J. ; Siepel, Adam ; Lis, John T. ; Kraus, W. Lee. / A rapid, extensive, and transient transcriptional response to estrogen signaling in breast cancer cells. In: Cell. 2011 ; Vol. 145, No. 4. pp. 622-634.
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