A rechargeable anti-thrombotic coating for blood-contacting devices

Hyun Ok Ham, Carolyn A. Haller, Guowei Su, Erbin Dai, Madhukar S. Patel, David R. Liu, Jian Liu, Elliot L. Chaikof

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Despite the potential of anti-thrombogenic coatings, including heparinized surfaces, to improve the performance of blood-contacting devices, the inevitable deterioration of bioactivity remains an important factor in device failure and related thrombotic complications. As a consequence, the ability to restore the bioactivity of a surface coating after implantation of a blood-contacting device provides a potentially important strategy to enhance its clinical performance. Here, we report the regeneration of a multicomponent anti-thrombogenic coating through use of an evolved sortase A to mediate reversible transpeptidation. Both recombinant thrombomodulin and a chemoenzymatically synthesized ultra-low molecular weight heparin were repeatedly and selectively immobilized or removed in a sequential, alternating, or simultaneous manner. The generation of activated protein C (aPC) and inhibition of activated factor X (FXa) was consistent with the molecular composition of the surface. The fabrication of a rechargeable anti-thrombogenic surface was demonstrated on an expanded polytetrafluoroethylene (ePTFE) vascular graft with reconstitution of the surface bound coating 4 weeks after in vivo implantation in a rat model.

Original languageEnglish (US)
Article number121011
JournalBiomaterials
Volume276
DOIs
StatePublished - Sep 2021
Externally publishedYes

Keywords

  • Anti-thrombogenic coating
  • Bioactive surface
  • Blood-contacting devices
  • Site-specific bioconjugation
  • Ultralow molecular weight heparin
  • Vascular graft

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Ceramics and Composites
  • Biomaterials
  • Mechanics of Materials

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