A role for coactivators and histone acetylation in estrogen receptor α-mediated transcription initiation

Mi Young Kim, Susan J. Hsiao, W. Lee Kraus

Research output: Contribution to journalArticlepeer-review

82 Scopus citations

Abstract

Transcriptional regulation by estrogen receptor α (ERα) involves protein-protein interactions among the receptor, its associated coactivators and the RNA polymerase II transcriptional machinery. We have used an in vitro chromatin assembly and transcription system to examine the biochemistry of interactions among ERα, the SRC proteins and p300/CBP. Using polypeptides designed to block specific receptor-cofactor or cofactor-cofactor interactions, we show that interactions among ERα, its coactivators and the RNA pol II machinery are all required for ERα-mediated transcription. Furthermore, we show that ERα-SRC-p300/CBP interactions are necessary and sufficient for the targeted acetylation of nucleosomal histones on estrogen-responsive promoters in the absence of transcription. The protein-protein interactions required for histone acetylation constitute a subset of the interactions required for transcriptional activation. Finally, we show that the major role of SRC-p300/CBP interactions is to enhance ERα-mediated transcription initiation, and they have little or no role in stimulating subsequent rounds of transcription. Together, our results indicate a specific role for the SRC and p300/CBP coactivators, as well as targeted histone acetylation, in ERα-mediated transcription.

Original languageEnglish (US)
Pages (from-to)6084-6094
Number of pages11
JournalEMBO Journal
Volume20
Issue number21
DOIs
StatePublished - Nov 1 2001

Keywords

  • Chromatin
  • Coactivator
  • Estrogen receptor
  • Histone acetylation
  • Transcription

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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