A role for NF-κB-dependent gene transactivation in sunburn

Kazuhiro Abeyama, William Eng, James V. Jester, Arie A. Vink, Dale Edelbaum, Clay J. Cockerell, Paul R. Bergstresser, Akira Takashima

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Exposure of skin to ultraviolet (UV) radiation is known to induce NF-κB activation, but the functional role for this pathway in UV-induced cutaneous inflammation remains uncertain. In this study, we examined whether experimentally induced sunburn reactions in mice could be prevented by blocking UV-induced, NF-κB-dependent gene transactivation with oligodeoxynucleotides (ODNs) containing the NF-κB cis element (NF-κB decoy ODNs). UV-induced secretion of IL-1, IL-6, TNF-α, and VEGF by skin-derived cell lines was inhibited by the decoy ODNs, but not by the scrambled control ODNs. Systemic or local injection of NF-κB decoy ODNs also inhibited cutaneous swelling responses to UV irradiation. Moreover, local UV-induced inflammatory changes (swelling, leukocyte infiltration, epidermal hyperplasia, and accumulation of proinflammatory cytokines) were all inhibited specifically by topically applied decoy ODNs. Importantly, these ODNs had no effect on alternative types of cutaneous inflammation caused by irritant or allergic chemicals. These results indicate that sunburn reactions culminate from inflammatory events that are triggered by UV-activated transcription of NF-κB target genes, rather than from nonspecific changes associated with tissue damage.

Original languageEnglish (US)
Pages (from-to)1751-1759
Number of pages9
JournalJournal of Clinical Investigation
Volume105
Issue number12
StatePublished - 2000

Fingerprint

Sunburn
Oligodeoxyribonucleotides
Transcriptional Activation
Genes
Skin
Inflammation
Irritants
Interleukin-1
Vascular Endothelial Growth Factor A
Hyperplasia
Interleukin-6
Leukocytes
Radiation
Cytokines
Cell Line
Injections

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Abeyama, K., Eng, W., Jester, J. V., Vink, A. A., Edelbaum, D., Cockerell, C. J., ... Takashima, A. (2000). A role for NF-κB-dependent gene transactivation in sunburn. Journal of Clinical Investigation, 105(12), 1751-1759.

A role for NF-κB-dependent gene transactivation in sunburn. / Abeyama, Kazuhiro; Eng, William; Jester, James V.; Vink, Arie A.; Edelbaum, Dale; Cockerell, Clay J.; Bergstresser, Paul R.; Takashima, Akira.

In: Journal of Clinical Investigation, Vol. 105, No. 12, 2000, p. 1751-1759.

Research output: Contribution to journalArticle

Abeyama, K, Eng, W, Jester, JV, Vink, AA, Edelbaum, D, Cockerell, CJ, Bergstresser, PR & Takashima, A 2000, 'A role for NF-κB-dependent gene transactivation in sunburn', Journal of Clinical Investigation, vol. 105, no. 12, pp. 1751-1759.
Abeyama K, Eng W, Jester JV, Vink AA, Edelbaum D, Cockerell CJ et al. A role for NF-κB-dependent gene transactivation in sunburn. Journal of Clinical Investigation. 2000;105(12):1751-1759.
Abeyama, Kazuhiro ; Eng, William ; Jester, James V. ; Vink, Arie A. ; Edelbaum, Dale ; Cockerell, Clay J. ; Bergstresser, Paul R. ; Takashima, Akira. / A role for NF-κB-dependent gene transactivation in sunburn. In: Journal of Clinical Investigation. 2000 ; Vol. 105, No. 12. pp. 1751-1759.
@article{e886a7fbcb0b4c52b96b6bd40bebdb3d,
title = "A role for NF-κB-dependent gene transactivation in sunburn",
abstract = "Exposure of skin to ultraviolet (UV) radiation is known to induce NF-κB activation, but the functional role for this pathway in UV-induced cutaneous inflammation remains uncertain. In this study, we examined whether experimentally induced sunburn reactions in mice could be prevented by blocking UV-induced, NF-κB-dependent gene transactivation with oligodeoxynucleotides (ODNs) containing the NF-κB cis element (NF-κB decoy ODNs). UV-induced secretion of IL-1, IL-6, TNF-α, and VEGF by skin-derived cell lines was inhibited by the decoy ODNs, but not by the scrambled control ODNs. Systemic or local injection of NF-κB decoy ODNs also inhibited cutaneous swelling responses to UV irradiation. Moreover, local UV-induced inflammatory changes (swelling, leukocyte infiltration, epidermal hyperplasia, and accumulation of proinflammatory cytokines) were all inhibited specifically by topically applied decoy ODNs. Importantly, these ODNs had no effect on alternative types of cutaneous inflammation caused by irritant or allergic chemicals. These results indicate that sunburn reactions culminate from inflammatory events that are triggered by UV-activated transcription of NF-κB target genes, rather than from nonspecific changes associated with tissue damage.",
author = "Kazuhiro Abeyama and William Eng and Jester, {James V.} and Vink, {Arie A.} and Dale Edelbaum and Cockerell, {Clay J.} and Bergstresser, {Paul R.} and Akira Takashima",
year = "2000",
language = "English (US)",
volume = "105",
pages = "1751--1759",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "12",

}

TY - JOUR

T1 - A role for NF-κB-dependent gene transactivation in sunburn

AU - Abeyama, Kazuhiro

AU - Eng, William

AU - Jester, James V.

AU - Vink, Arie A.

AU - Edelbaum, Dale

AU - Cockerell, Clay J.

AU - Bergstresser, Paul R.

AU - Takashima, Akira

PY - 2000

Y1 - 2000

N2 - Exposure of skin to ultraviolet (UV) radiation is known to induce NF-κB activation, but the functional role for this pathway in UV-induced cutaneous inflammation remains uncertain. In this study, we examined whether experimentally induced sunburn reactions in mice could be prevented by blocking UV-induced, NF-κB-dependent gene transactivation with oligodeoxynucleotides (ODNs) containing the NF-κB cis element (NF-κB decoy ODNs). UV-induced secretion of IL-1, IL-6, TNF-α, and VEGF by skin-derived cell lines was inhibited by the decoy ODNs, but not by the scrambled control ODNs. Systemic or local injection of NF-κB decoy ODNs also inhibited cutaneous swelling responses to UV irradiation. Moreover, local UV-induced inflammatory changes (swelling, leukocyte infiltration, epidermal hyperplasia, and accumulation of proinflammatory cytokines) were all inhibited specifically by topically applied decoy ODNs. Importantly, these ODNs had no effect on alternative types of cutaneous inflammation caused by irritant or allergic chemicals. These results indicate that sunburn reactions culminate from inflammatory events that are triggered by UV-activated transcription of NF-κB target genes, rather than from nonspecific changes associated with tissue damage.

AB - Exposure of skin to ultraviolet (UV) radiation is known to induce NF-κB activation, but the functional role for this pathway in UV-induced cutaneous inflammation remains uncertain. In this study, we examined whether experimentally induced sunburn reactions in mice could be prevented by blocking UV-induced, NF-κB-dependent gene transactivation with oligodeoxynucleotides (ODNs) containing the NF-κB cis element (NF-κB decoy ODNs). UV-induced secretion of IL-1, IL-6, TNF-α, and VEGF by skin-derived cell lines was inhibited by the decoy ODNs, but not by the scrambled control ODNs. Systemic or local injection of NF-κB decoy ODNs also inhibited cutaneous swelling responses to UV irradiation. Moreover, local UV-induced inflammatory changes (swelling, leukocyte infiltration, epidermal hyperplasia, and accumulation of proinflammatory cytokines) were all inhibited specifically by topically applied decoy ODNs. Importantly, these ODNs had no effect on alternative types of cutaneous inflammation caused by irritant or allergic chemicals. These results indicate that sunburn reactions culminate from inflammatory events that are triggered by UV-activated transcription of NF-κB target genes, rather than from nonspecific changes associated with tissue damage.

UR - http://www.scopus.com/inward/record.url?scp=0033625503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033625503&partnerID=8YFLogxK

M3 - Article

VL - 105

SP - 1751

EP - 1759

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 12

ER -