A Role for the CHC22 clathrin heavy-chain isoform in human glucose metabolism

Stéphane Vassilopoulos, Christopher Esk, Sachiko Hoshino, Birgit H. Funke, Chih Ying Chen, Alex M. Plocik, Woodring E. Wright, Raju Kucherlapati, Frances M. Brodsky

Research output: Contribution to journalArticle

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Abstract

Intracellular trafficking of the glucose transporter GLUT4 from storage compartments to the plasma membrane is triggered in muscle and fat during the body's response to insulin. Clathrin is involved in intracellular trafficking, and in humans, the clathrin heavy-chain isoform CHC22 is highly expressed in skeletal muscle. We found a role for CHC22 in the formation of insulin-responsive GLUT4 compartments in human muscle and adipocytes. CHC22 also associated with expanded GLUT4 compartments in muscle from type 2 diabetic patients. Tissue-specific introduction of CHC22 in mice, which have only a pseudogene for this protein, caused aberrant localization of GLUT4 transport pathway components in their muscle, as well as features of diabetes. Thus, CHC22-dependent membrane trafficking constitutes a species-restricted pathway in human muscle and fat with potential implications for type 2 diabetes.

Original languageEnglish (US)
Pages (from-to)1192-1196
Number of pages5
JournalScience
Volume324
Issue number5931
DOIs
StatePublished - May 29 2009

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Clathrin Heavy Chains
Protein Isoforms
Glucose
Muscles
Human Trafficking
Fats
Insulin
Clathrin
Pseudogenes
Facilitative Glucose Transport Proteins
Adipocytes
Type 2 Diabetes Mellitus
Skeletal Muscle
Cell Membrane
Membranes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Vassilopoulos, S., Esk, C., Hoshino, S., Funke, B. H., Chen, C. Y., Plocik, A. M., ... Brodsky, F. M. (2009). A Role for the CHC22 clathrin heavy-chain isoform in human glucose metabolism. Science, 324(5931), 1192-1196. https://doi.org/10.1126/science.1171529

A Role for the CHC22 clathrin heavy-chain isoform in human glucose metabolism. / Vassilopoulos, Stéphane; Esk, Christopher; Hoshino, Sachiko; Funke, Birgit H.; Chen, Chih Ying; Plocik, Alex M.; Wright, Woodring E.; Kucherlapati, Raju; Brodsky, Frances M.

In: Science, Vol. 324, No. 5931, 29.05.2009, p. 1192-1196.

Research output: Contribution to journalArticle

Vassilopoulos, S, Esk, C, Hoshino, S, Funke, BH, Chen, CY, Plocik, AM, Wright, WE, Kucherlapati, R & Brodsky, FM 2009, 'A Role for the CHC22 clathrin heavy-chain isoform in human glucose metabolism', Science, vol. 324, no. 5931, pp. 1192-1196. https://doi.org/10.1126/science.1171529
Vassilopoulos S, Esk C, Hoshino S, Funke BH, Chen CY, Plocik AM et al. A Role for the CHC22 clathrin heavy-chain isoform in human glucose metabolism. Science. 2009 May 29;324(5931):1192-1196. https://doi.org/10.1126/science.1171529
Vassilopoulos, Stéphane ; Esk, Christopher ; Hoshino, Sachiko ; Funke, Birgit H. ; Chen, Chih Ying ; Plocik, Alex M. ; Wright, Woodring E. ; Kucherlapati, Raju ; Brodsky, Frances M. / A Role for the CHC22 clathrin heavy-chain isoform in human glucose metabolism. In: Science. 2009 ; Vol. 324, No. 5931. pp. 1192-1196.
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