A role for the Drosophila Bag-of-marbles protein in the differentiation of cystoblasts from germline stem cells

D. McKearin, B. Ohlstein

Research output: Contribution to journalArticlepeer-review

248 Scopus citations

Abstract

Cell differentiation commonly dictates a change in the cell cycle of mitotic daughters. Previous investigations have suggested that the Drosophila bag of marbles (bam) gene is required for the differentiation of germline stem cell daughters (cystoblasts) from the mother stem cells, perhaps by altering the cell cycle. In this paper, we report the preparation of antibodies to the Bam protein and the use of those reagents to investigate how Bam is required for germ cell development. We find that Bam exists as both a fusome component and as cytoplasmic protein and that cytoplasmic and fusome Bam might have separable activities. We also show that barn mutant germ cells are blocked in differentiation and are trapped as mitotically active cells like stem cells. A model for how Bam might regulate cystocyte differentiation is presented.

Original languageEnglish (US)
Pages (from-to)2937-2947
Number of pages11
JournalDevelopment
Volume121
Issue number9
StatePublished - 1995

Keywords

  • Bag of marbles
  • Cell cycle
  • Cytoblast
  • Drosophila
  • Fusome
  • Germ cell
  • Mitosis
  • Stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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