TY - JOUR
T1 - A role for the Ral guanine nucleotide dissociation stimulator in mediating Ras-induced transformation
AU - White, Michael A.
AU - Vale, Terry
AU - Camonis, Jacques H.
AU - Schaefer, Erik
AU - Wigler, Michael H.
PY - 1996
Y1 - 1996
N2 - Oncogenic Ras transforms cells through the activation of multiple downstream pathways mediated by separate effector molecules, one of which is Raf. Here we report the identification era second ras-binding protein that can induce cellular transformation in parallel with activation of the Raf/mitogen-activated protein kinase cascade. The Ral guanine nucleotide dissociation stimulator (RalGDS) was isolated from a screen for Ras-binding proteins that specifically interact with a Ras effector-loop mutant, ras(12V,37G), that uncouples Ras from activation of Raf1. RalGDS, like ras(12V,37G), cooperates synergistically with mutationally activated Raf to induce foci of growth and morphologically transformed NIH 3T3 cells. RalGDS does not significantly enhance MAP kinase activation by activated Raf, suggesting that the cooperativity in focus formation is due to a distinct pathway acting downstream of Ras and parallel to Raf.
AB - Oncogenic Ras transforms cells through the activation of multiple downstream pathways mediated by separate effector molecules, one of which is Raf. Here we report the identification era second ras-binding protein that can induce cellular transformation in parallel with activation of the Raf/mitogen-activated protein kinase cascade. The Ral guanine nucleotide dissociation stimulator (RalGDS) was isolated from a screen for Ras-binding proteins that specifically interact with a Ras effector-loop mutant, ras(12V,37G), that uncouples Ras from activation of Raf1. RalGDS, like ras(12V,37G), cooperates synergistically with mutationally activated Raf to induce foci of growth and morphologically transformed NIH 3T3 cells. RalGDS does not significantly enhance MAP kinase activation by activated Raf, suggesting that the cooperativity in focus formation is due to a distinct pathway acting downstream of Ras and parallel to Raf.
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U2 - 10.1074/jbc.271.28.16439
DO - 10.1074/jbc.271.28.16439
M3 - Article
C2 - 8663585
AN - SCOPUS:0029950722
SN - 0021-9258
VL - 271
SP - 16439
EP - 16442
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 28
ER -