Abstract
The transcriptional coactivator peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α) has been identified as an inducible regulator of mitochondrial function. Skeletal muscle PGC-1α expression is induced post-exercise. Therefore, we sought to determine its role in the regulation of muscle fuel metabolism. Studies were performed using conditional, muscle-specific, PGC-1α gain-of-function and constitutive, generalized, loss-of-function mice. Forced expression of PGC-1α increased muscle glucose uptake concomitant with augmentation of glycogen stores, a metabolic response similar to postexercise recovery. Induction of muscle PGC-1α expression prevented muscle glycogen depletion during exercise. Conversely, PGC-1α-deficient animals exhibited reduced rates of muscle glycogen repletion post-exercise. PGC-1α was shown to increase muscle glycogen stores via several mechanisms including stimulation of glucose import, suppression of glycolytic flux, and by down-regulation of the expression of glycogen phosphorylase and its activating kinase, phosphorylase kinase α. These findings identify PGC-1α as a critical regulator of skeletal muscle fuel stores.
Original language | English (US) |
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Pages (from-to) | 36642-36651 |
Number of pages | 10 |
Journal | Journal of Biological Chemistry |
Volume | 282 |
Issue number | 50 |
DOIs | |
State | Published - Dec 14 2007 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology