A pulse scheme for measuring cross-correlation between 13Cα-1Hα dipolar and carbonyl chemical shift anisotropy relaxation mechanisms is presented from which the protein backbone dihedral angle ψ is measured. The method offers significant sensitivity gains relative to our recently published scheme for measuring ψ based on this cross-correlation effect [Yang et al. (1997) J. Am. Chem. Soc., 119, 11938-11940]. The utility of the method is demonstrated with an application to a 42 kDa complex of 15N,13C-labeled maltose binding protein and β-cyclodextrin.
- Chemical shift anisotropy
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