A sequestered fusion peptide in the structure of an HIV-1 transmitted founder envelope trimer

Neeti Ananthaswamy, Qianglin Fang, Wadad AlSalmi, Swati Jain, Zhenguo Chen, Thomas Klose, Yingyuan Sun, Yue Liu, Marthandan Mahalingam, Subhash Chand, Sodsai Tovanabutra, Merlin L. Robb, Michael G. Rossmann, Venigalla B. Rao

Research output: Contribution to journalArticle

Abstract

The envelope protein of human immunodeficiency virus-1 (HIV-1) and its fusion peptide are essential for cell entry and vaccine design. Here, we describe the 3.9-Å resolution structure of an envelope protein trimer from a very early transmitted founder virus (CRF01_AE T/F100) complexed with Fab from the broadly neutralizing antibody (bNAb) 8ANC195. The overall T/F100 trimer structure is similar to other reported “closed” state prefusion trimer structures. In contrast, the fusion peptide, which is exposed to solvent in reported closed structures, is sequestered (buried) in the hydrophobic core of the T/F100 trimer. A buried conformation has previously been observed in “open” state structures formed after CD4 receptor binding. The T/F100 trimer binds poorly to bNAbs including the fusion peptide-specific bNAbs PGT151 and VRC34.01. The T/F100 structure might represent a prefusion state, intermediate between the closed and open states. These observations are relevant to mechanisms of HIV-1 transmission and vaccine design.

Original languageEnglish (US)
Article number873
JournalNature Communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019
Externally publishedYes

Fingerprint

human immunodeficiency virus
trimers
Viruses
peptides
HIV-1
envelopes
Fusion reactions
fusion
Peptides
Human Immunodeficiency Virus env Gene Products
vaccines
Vaccines
CD4 Antigens
Neutralizing Antibodies
Conformations
Proteins
proteins
viruses
antibodies
entry

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Ananthaswamy, N., Fang, Q., AlSalmi, W., Jain, S., Chen, Z., Klose, T., ... Rao, V. B. (2019). A sequestered fusion peptide in the structure of an HIV-1 transmitted founder envelope trimer. Nature Communications, 10(1), [873]. https://doi.org/10.1038/s41467-019-08825-7

A sequestered fusion peptide in the structure of an HIV-1 transmitted founder envelope trimer. / Ananthaswamy, Neeti; Fang, Qianglin; AlSalmi, Wadad; Jain, Swati; Chen, Zhenguo; Klose, Thomas; Sun, Yingyuan; Liu, Yue; Mahalingam, Marthandan; Chand, Subhash; Tovanabutra, Sodsai; Robb, Merlin L.; Rossmann, Michael G.; Rao, Venigalla B.

In: Nature Communications, Vol. 10, No. 1, 873, 01.12.2019.

Research output: Contribution to journalArticle

Ananthaswamy, N, Fang, Q, AlSalmi, W, Jain, S, Chen, Z, Klose, T, Sun, Y, Liu, Y, Mahalingam, M, Chand, S, Tovanabutra, S, Robb, ML, Rossmann, MG & Rao, VB 2019, 'A sequestered fusion peptide in the structure of an HIV-1 transmitted founder envelope trimer', Nature Communications, vol. 10, no. 1, 873. https://doi.org/10.1038/s41467-019-08825-7
Ananthaswamy, Neeti ; Fang, Qianglin ; AlSalmi, Wadad ; Jain, Swati ; Chen, Zhenguo ; Klose, Thomas ; Sun, Yingyuan ; Liu, Yue ; Mahalingam, Marthandan ; Chand, Subhash ; Tovanabutra, Sodsai ; Robb, Merlin L. ; Rossmann, Michael G. ; Rao, Venigalla B. / A sequestered fusion peptide in the structure of an HIV-1 transmitted founder envelope trimer. In: Nature Communications. 2019 ; Vol. 10, No. 1.
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