A silver-stain modification of standard histological slide preparation for use in anthropology analyses

Deborrah C. Pinto, Eric D. Pace

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The traditional histology method typically employed by forensic anthropologists involves plastic embedding of undecalcified bone. The embedded sample is then cut by a diamond blade saw and ground to the required thickness of ~50-100 microns using a grinder. There are several limitations to this method: Cement lines may be blurred; depth-of-field artifacts may result from viewing thick sections; and medicolegal offices with limited budgets may not be able to invest in additional equipment or training for this method. A silver nitrate stain modification of the standard histology preparation technique of decalcified bone is presented. The benefits of this technique are that: Cement lines are viewed clearly; no depth-of-field artifacts are present; and because this is a modification of the standard technique used by histology laboratories typically employed by medicolegal offices, no additional equipment or training is required.

Original languageEnglish (US)
Pages (from-to)391-398
Number of pages8
JournalJournal of Forensic Sciences
Volume60
Issue number2
DOIs
StatePublished - Mar 1 2015
Externally publishedYes

Keywords

  • Bone histology
  • Cement lines
  • Forensic anthropology
  • Forensic science
  • Silver nitrate stain
  • Slide preparation method

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Genetics

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