A similar ribosomal protein S6 kinase activity is found in insulin-treated 3T3-L1 cells and chick embryo fibroblasts transformed by Rous sarcoma virus

Melanie H. Cobb; John G. Burr; Maurine E. Linder; Teri B. Gray; Jill S. Gregory

doi:10.1016/0006-291X(86)91135-6

A similar ribosomal protein S6 kinase activity is found in insulin-treated 3T3-L1 cells and chick embryo fibroblasts transformed by Rous sarcoma virus

Melanie H. Cobb, John G. Burr, Maurine E. Linder, Teri B. Gray, Jill S. Gregory

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Insulin and transformation by Rous sarcoma virus stimulate the phosphorylation of ribosomal protein S6. Soluble fractions containing activated S6 protein kinase from insulin-treated cells and from transformed chick embryo fibroblasts were compared. Based upon several characteristics notably elution from DEAE-cellulose and sedimentation in glycerol gradients, these two S6 protein kinase activities appear to be similar enzymes. Thus insulin and retroviral transformation may activate the same enzyme to regulate the phosphorylation state of S6.

Original language	English (US)
Pages (from-to)	702-708
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	137
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(86)91135-6
State	Published - Jun 13 1986

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(86)91135-6

Cite this

A similar ribosomal protein S6 kinase activity is found in insulin-treated 3T3-L1 cells and chick embryo fibroblasts transformed by Rous sarcoma virus. / Cobb, Melanie H.; Burr, John G.; Linder, Maurine E. et al.
In: Biochemical and Biophysical Research Communications, Vol. 137, No. 2, 13.06.1986, p. 702-708.

Research output: Contribution to journal › Article › peer-review

@article{fb7e87909e4348de88224477f4ab5872,

title = "A similar ribosomal protein S6 kinase activity is found in insulin-treated 3T3-L1 cells and chick embryo fibroblasts transformed by Rous sarcoma virus",

abstract = "Insulin and transformation by Rous sarcoma virus stimulate the phosphorylation of ribosomal protein S6. Soluble fractions containing activated S6 protein kinase from insulin-treated cells and from transformed chick embryo fibroblasts were compared. Based upon several characteristics notably elution from DEAE-cellulose and sedimentation in glycerol gradients, these two S6 protein kinase activities appear to be similar enzymes. Thus insulin and retroviral transformation may activate the same enzyme to regulate the phosphorylation state of S6.",

author = "Cobb, {Melanie H.} and Burr, {John G.} and Linder, {Maurine E.} and Gray, {Teri B.} and Gregory, {Jill S.}",

note = "Funding Information: Diabetes t This project Foundation was supported and grant by #AM a grant 34128 from from the the Institutes of Health (to M.H.C.) and by BRSG SO7 awarded by the Biomedical Research Support Grant Division of Research Resources, National Institutes Health (to J.G.B.).",

year = "1986",

month = jun,

day = "13",

doi = "10.1016/0006-291X(86)91135-6",

language = "English (US)",

volume = "137",

pages = "702--708",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - A similar ribosomal protein S6 kinase activity is found in insulin-treated 3T3-L1 cells and chick embryo fibroblasts transformed by Rous sarcoma virus

AU - Cobb, Melanie H.

AU - Burr, John G.

AU - Linder, Maurine E.

AU - Gray, Teri B.

AU - Gregory, Jill S.

N1 - Funding Information: Diabetes t This project Foundation was supported and grant by #AM a grant 34128 from from the the Institutes of Health (to M.H.C.) and by BRSG SO7 awarded by the Biomedical Research Support Grant Division of Research Resources, National Institutes Health (to J.G.B.).

PY - 1986/6/13

Y1 - 1986/6/13

N2 - Insulin and transformation by Rous sarcoma virus stimulate the phosphorylation of ribosomal protein S6. Soluble fractions containing activated S6 protein kinase from insulin-treated cells and from transformed chick embryo fibroblasts were compared. Based upon several characteristics notably elution from DEAE-cellulose and sedimentation in glycerol gradients, these two S6 protein kinase activities appear to be similar enzymes. Thus insulin and retroviral transformation may activate the same enzyme to regulate the phosphorylation state of S6.

AB - Insulin and transformation by Rous sarcoma virus stimulate the phosphorylation of ribosomal protein S6. Soluble fractions containing activated S6 protein kinase from insulin-treated cells and from transformed chick embryo fibroblasts were compared. Based upon several characteristics notably elution from DEAE-cellulose and sedimentation in glycerol gradients, these two S6 protein kinase activities appear to be similar enzymes. Thus insulin and retroviral transformation may activate the same enzyme to regulate the phosphorylation state of S6.

UR - http://www.scopus.com/inward/record.url?scp=0022462285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022462285&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(86)91135-6

DO - 10.1016/0006-291X(86)91135-6

M3 - Article

C2 - 3015127

AN - SCOPUS:0022462285

SN - 0006-291X

VL - 137

SP - 702

EP - 708

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

A similar ribosomal protein S6 kinase activity is found in insulin-treated 3T3-L1 cells and chick embryo fibroblasts transformed by Rous sarcoma virus

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this