A simple, one-step fluorometric method for determination of nanomolar concentrations of terbium

Thomas D. Barela, A. Dean Sherry

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

A simple, one-step spectrofluorometric method for determination of terbium is described. The technique requires a single addition of dipicolinic acid to a terbium-containing solution, is sensitive to 10 nm Tb3+, and is independent of pH in the range 5.0-10.5. The method, applicable in several buffer systems and in the presence of large concentrations of alkali metals, is applied to the determination of Tb3+-apoconcanavalin A association constants.

Original languageEnglish (US)
Pages (from-to)351-352
Number of pages2
JournalAnalytical biochemistry
Volume71
Issue number2
DOIs
StatePublished - Apr 1976

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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