A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution

Richard K. Bruick, Marcus Koppitz, Gerald F. Joyce, Leslie E. Orgel

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A rapid method for the synthesis of oligodeoxynucleotides (ODNs) terminated by 5'-amino-5'-deoxythymidine is described. A 3'-phosphorylated ODN (the donor) is incubated in aqueous solution with 5'-amino-5'-deoxythymidine in the presence of N-(3-dimethyl-aminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), extending the donor by one residue via a phosphoramidate bond. Template- directed ligation of the extended donor and an acceptor ODN, followed by acid hydrolysis, yields the acceptor ODN extended by a single 5'-amino-5'-deoxythymidine residue at its 5' terminus.

Original languageEnglish (US)
Pages (from-to)1309-1310
Number of pages2
JournalNucleic acids research
Volume25
Issue number6
DOIs
StatePublished - Mar 15 1997

ASJC Scopus subject areas

  • Genetics

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