A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution

Richard K. Bruick, Marcus Koppitz, Gerald F. Joyce, Leslie E. Orgel

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A rapid method for the synthesis of oligodeoxynucleotides (ODNs) terminated by 5'-amino-5'-deoxythymidine is described. A 3'-phosphorylated ODN (the donor) is incubated in aqueous solution with 5'-amino-5'-deoxythymidine in the presence of N-(3-dimethyl-aminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), extending the donor by one residue via a phosphoramidate bond. Template- directed ligation of the extended donor and an acceptor ODN, followed by acid hydrolysis, yields the acceptor ODN extended by a single 5'-amino-5'-deoxythymidine residue at its 5' terminus.

Original languageEnglish (US)
Pages (from-to)1309-1310
Number of pages2
JournalNucleic Acids Research
Volume25
Issue number6
DOIs
StatePublished - Mar 15 1997

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Oligodeoxyribonucleotides
Ligation
Hydrolysis
Acids
5'-amino-5'-deoxythymidine

ASJC Scopus subject areas

  • Genetics

Cite this

A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution. / Bruick, Richard K.; Koppitz, Marcus; Joyce, Gerald F.; Orgel, Leslie E.

In: Nucleic Acids Research, Vol. 25, No. 6, 15.03.1997, p. 1309-1310.

Research output: Contribution to journalArticle

Bruick, Richard K. ; Koppitz, Marcus ; Joyce, Gerald F. ; Orgel, Leslie E. / A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution. In: Nucleic Acids Research. 1997 ; Vol. 25, No. 6. pp. 1309-1310.
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