A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution

Richard K. Bruick; Marcus Koppitz; Gerald F. Joyce; Leslie E. Orgel

doi:10.1093/nar/25.6.1309

A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution

Richard K. Bruick, Marcus Koppitz, Gerald F. Joyce, Leslie E. Orgel

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A rapid method for the synthesis of oligodeoxynucleotides (ODNs) terminated by 5'-amino-5'-deoxythymidine is described. A 3'-phosphorylated ODN (the donor) is incubated in aqueous solution with 5'-amino-5'-deoxythymidine in the presence of N-(3-dimethyl-aminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), extending the donor by one residue via a phosphoramidate bond. Template- directed ligation of the extended donor and an acceptor ODN, followed by acid hydrolysis, yields the acceptor ODN extended by a single 5'-amino-5'-deoxythymidine residue at its 5' terminus.

Original language	English (US)
Pages (from-to)	1309-1310
Number of pages	2
Journal	Nucleic acids research
Volume	25
Issue number	6
DOIs	https://doi.org/10.1093/nar/25.6.1309
State	Published - Mar 15 1997

ASJC Scopus subject areas

Genetics

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10.1093/nar/25.6.1309

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title = "A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution",

abstract = "A rapid method for the synthesis of oligodeoxynucleotides (ODNs) terminated by 5'-amino-5'-deoxythymidine is described. A 3'-phosphorylated ODN (the donor) is incubated in aqueous solution with 5'-amino-5'-deoxythymidine in the presence of N-(3-dimethyl-aminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), extending the donor by one residue via a phosphoramidate bond. Template- directed ligation of the extended donor and an acceptor ODN, followed by acid hydrolysis, yields the acceptor ODN extended by a single 5'-amino-5'-deoxythymidine residue at its 5' terminus.",

author = "Bruick, {Richard K.} and Marcus Koppitz and Joyce, {Gerald F.} and Orgel, {Leslie E.}",

note = "Funding Information: This work was supported by the NASA Specialized Center for Research and Training (NSCORT) in Exobiology (grant #NAGW-2881).",

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doi = "10.1093/nar/25.6.1309",

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T1 - A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution

AU - Bruick, Richard K.

AU - Koppitz, Marcus

AU - Joyce, Gerald F.

AU - Orgel, Leslie E.

N1 - Funding Information: This work was supported by the NASA Specialized Center for Research and Training (NSCORT) in Exobiology (grant #NAGW-2881).

PY - 1997/3/15

Y1 - 1997/3/15

N2 - A rapid method for the synthesis of oligodeoxynucleotides (ODNs) terminated by 5'-amino-5'-deoxythymidine is described. A 3'-phosphorylated ODN (the donor) is incubated in aqueous solution with 5'-amino-5'-deoxythymidine in the presence of N-(3-dimethyl-aminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), extending the donor by one residue via a phosphoramidate bond. Template- directed ligation of the extended donor and an acceptor ODN, followed by acid hydrolysis, yields the acceptor ODN extended by a single 5'-amino-5'-deoxythymidine residue at its 5' terminus.

AB - A rapid method for the synthesis of oligodeoxynucleotides (ODNs) terminated by 5'-amino-5'-deoxythymidine is described. A 3'-phosphorylated ODN (the donor) is incubated in aqueous solution with 5'-amino-5'-deoxythymidine in the presence of N-(3-dimethyl-aminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), extending the donor by one residue via a phosphoramidate bond. Template- directed ligation of the extended donor and an acceptor ODN, followed by acid hydrolysis, yields the acceptor ODN extended by a single 5'-amino-5'-deoxythymidine residue at its 5' terminus.

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A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution

Abstract

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