A simplified method for purification of human C5a from citrated plasma

Lois Renfer, Michael M. Frank, Carl H. Hammer, Liana Harvath, Thomas J. Lawley, Kim B. Yancey

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A simplified immunoadsorption technique has been developed to purify human C5a, the 11 000 Da glycopeptide produced by C5 convertase cleavage of the fifth component of complement. In this method, human C5 fragments, including C5a, are isolated from zymosan-activated plasma by affinity chromatography, concentrated on CM 52 cellulose, and then purified to homogeneity by gel filtration on Sephadex G-75 in phosphate-buffered saline. Human C5a prepared by this technique demonstrates characteristic immunochemical and biologic activity. This method has also been adapted for the purification of 125I-C5a in phosphate-buffered saline. This technique offers a simplified approach to the purification of this important soluble mediator of inflammation.

Original languageEnglish (US)
Pages (from-to)193-205
Number of pages13
JournalJournal of Immunological Methods
Volume88
Issue number2
DOIs
StatePublished - Apr 17 1986

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Complement C3-C5 Convertases
Phosphates
Inflammation Mediators
Zymosan
Glycopeptides
Affinity Chromatography
Cellulose
Gel Chromatography
sephadex

Keywords

  • Anaphylatoxin purification
  • Complement
  • Human C5a

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

A simplified method for purification of human C5a from citrated plasma. / Renfer, Lois; Frank, Michael M.; Hammer, Carl H.; Harvath, Liana; Lawley, Thomas J.; Yancey, Kim B.

In: Journal of Immunological Methods, Vol. 88, No. 2, 17.04.1986, p. 193-205.

Research output: Contribution to journalArticle

Renfer, Lois ; Frank, Michael M. ; Hammer, Carl H. ; Harvath, Liana ; Lawley, Thomas J. ; Yancey, Kim B. / A simplified method for purification of human C5a from citrated plasma. In: Journal of Immunological Methods. 1986 ; Vol. 88, No. 2. pp. 193-205.
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