A single antigen-specific B cell can conjugate to either a type 1 or a type 2 helper T cell

V. M. Sanders; R. Fernandez-Botran; R. L. Coffman; T. R. Mosmann; E. S. Vitetta

doi:10.1073/pnas.85.20.7724

A single antigen-specific B cell can conjugate to either a type 1 or a type 2 helper T cell

V. M. Sanders, R. Fernandez-Botran, R. L. Coffman, T. R. Mosmann, E. S. Vitetta

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Keyhole-limpet-hemocyanin-specific I-A(d)-restricted T helper cells type 1 (T(H1) cells) and type 2 (T(H2) cells) were studied for their ability to physically conjugate to trinitrophenyl-specific antigen-binding B cells (TNP-ABCs). The same TNP-ABCs (which had already processed TNP-keyhole limpet hemocyanin) formed conjugates with one or the other T helper (T(H)) cell type, and conjugation was antigen-specific and major histocompatibility complex-restricted. The conjugation of a fixed number of one type of T(H) cell to the TNP-ABCs was inhibited by co-incubation with increasing numbers of cells of the other T(H) cell type. Thus, the vast majority of TNP-ABCs can conjugate to either type of T(H) cell. A similar pattern of inhibition of the conjugation of one T(H) cell type to the TNP-ABCs was seen when cells were co-incubated with increasing numbers of cells from an alloreactive T-cell clone. In all cases, conjugates contained only one T cell bound to one B cell, suggesting that interaction of an antigen-presenting B cell with one T(H) cell prevents the simultaneous binding of another T(H) cell.

Original language	English (US)
Pages (from-to)	7724-7728
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	85
Issue number	20
DOIs	https://doi.org/10.1073/pnas.85.20.7724
State	Published - 1988

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.85.20.7724

Fingerprint Dive into the research topics of 'A single antigen-specific B cell can conjugate to either a type 1 or a type 2 helper T cell'. Together they form a unique fingerprint.

Cite this

@article{9ad22bada4d943318a3c0693e0dd3e9f,

title = "A single antigen-specific B cell can conjugate to either a type 1 or a type 2 helper T cell",

abstract = "Keyhole-limpet-hemocyanin-specific I-A(d)-restricted T helper cells type 1 (T(H1) cells) and type 2 (T(H2) cells) were studied for their ability to physically conjugate to trinitrophenyl-specific antigen-binding B cells (TNP-ABCs). The same TNP-ABCs (which had already processed TNP-keyhole limpet hemocyanin) formed conjugates with one or the other T helper (T(H)) cell type, and conjugation was antigen-specific and major histocompatibility complex-restricted. The conjugation of a fixed number of one type of T(H) cell to the TNP-ABCs was inhibited by co-incubation with increasing numbers of cells of the other T(H) cell type. Thus, the vast majority of TNP-ABCs can conjugate to either type of T(H) cell. A similar pattern of inhibition of the conjugation of one T(H) cell type to the TNP-ABCs was seen when cells were co-incubated with increasing numbers of cells from an alloreactive T-cell clone. In all cases, conjugates contained only one T cell bound to one B cell, suggesting that interaction of an antigen-presenting B cell with one T(H) cell prevents the simultaneous binding of another T(H) cell.",

author = "Sanders, {V. M.} and R. Fernandez-Botran and Coffman, {R. L.} and Mosmann, {T. R.} and Vitetta, {E. S.}",

year = "1988",

doi = "10.1073/pnas.85.20.7724",

language = "English (US)",

volume = "85",

pages = "7724--7728",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "20",

}

TY - JOUR

T1 - A single antigen-specific B cell can conjugate to either a type 1 or a type 2 helper T cell

AU - Sanders, V. M.

AU - Fernandez-Botran, R.

AU - Coffman, R. L.

AU - Mosmann, T. R.

AU - Vitetta, E. S.

PY - 1988

Y1 - 1988

N2 - Keyhole-limpet-hemocyanin-specific I-A(d)-restricted T helper cells type 1 (T(H1) cells) and type 2 (T(H2) cells) were studied for their ability to physically conjugate to trinitrophenyl-specific antigen-binding B cells (TNP-ABCs). The same TNP-ABCs (which had already processed TNP-keyhole limpet hemocyanin) formed conjugates with one or the other T helper (T(H)) cell type, and conjugation was antigen-specific and major histocompatibility complex-restricted. The conjugation of a fixed number of one type of T(H) cell to the TNP-ABCs was inhibited by co-incubation with increasing numbers of cells of the other T(H) cell type. Thus, the vast majority of TNP-ABCs can conjugate to either type of T(H) cell. A similar pattern of inhibition of the conjugation of one T(H) cell type to the TNP-ABCs was seen when cells were co-incubated with increasing numbers of cells from an alloreactive T-cell clone. In all cases, conjugates contained only one T cell bound to one B cell, suggesting that interaction of an antigen-presenting B cell with one T(H) cell prevents the simultaneous binding of another T(H) cell.

AB - Keyhole-limpet-hemocyanin-specific I-A(d)-restricted T helper cells type 1 (T(H1) cells) and type 2 (T(H2) cells) were studied for their ability to physically conjugate to trinitrophenyl-specific antigen-binding B cells (TNP-ABCs). The same TNP-ABCs (which had already processed TNP-keyhole limpet hemocyanin) formed conjugates with one or the other T helper (T(H)) cell type, and conjugation was antigen-specific and major histocompatibility complex-restricted. The conjugation of a fixed number of one type of T(H) cell to the TNP-ABCs was inhibited by co-incubation with increasing numbers of cells of the other T(H) cell type. Thus, the vast majority of TNP-ABCs can conjugate to either type of T(H) cell. A similar pattern of inhibition of the conjugation of one T(H) cell type to the TNP-ABCs was seen when cells were co-incubated with increasing numbers of cells from an alloreactive T-cell clone. In all cases, conjugates contained only one T cell bound to one B cell, suggesting that interaction of an antigen-presenting B cell with one T(H) cell prevents the simultaneous binding of another T(H) cell.

UR - http://www.scopus.com/inward/record.url?scp=0023692086&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023692086&partnerID=8YFLogxK

U2 - 10.1073/pnas.85.20.7724

DO - 10.1073/pnas.85.20.7724

M3 - Article

C2 - 2459715

AN - SCOPUS:0023692086

VL - 85

SP - 7724

EP - 7728

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 20

ER -