TY - JOUR
T1 - A single antigen-specific B cell can conjugate to either a type 1 or a type 2 helper T cell
AU - Sanders, V. M.
AU - Fernandez-Botran, R.
AU - Coffman, R. L.
AU - Mosmann, T. R.
AU - Vitetta, E. S.
PY - 1988
Y1 - 1988
N2 - Keyhole-limpet-hemocyanin-specific I-A(d)-restricted T helper cells type 1 (T(H1) cells) and type 2 (T(H2) cells) were studied for their ability to physically conjugate to trinitrophenyl-specific antigen-binding B cells (TNP-ABCs). The same TNP-ABCs (which had already processed TNP-keyhole limpet hemocyanin) formed conjugates with one or the other T helper (T(H)) cell type, and conjugation was antigen-specific and major histocompatibility complex-restricted. The conjugation of a fixed number of one type of T(H) cell to the TNP-ABCs was inhibited by co-incubation with increasing numbers of cells of the other T(H) cell type. Thus, the vast majority of TNP-ABCs can conjugate to either type of T(H) cell. A similar pattern of inhibition of the conjugation of one T(H) cell type to the TNP-ABCs was seen when cells were co-incubated with increasing numbers of cells from an alloreactive T-cell clone. In all cases, conjugates contained only one T cell bound to one B cell, suggesting that interaction of an antigen-presenting B cell with one T(H) cell prevents the simultaneous binding of another T(H) cell.
AB - Keyhole-limpet-hemocyanin-specific I-A(d)-restricted T helper cells type 1 (T(H1) cells) and type 2 (T(H2) cells) were studied for their ability to physically conjugate to trinitrophenyl-specific antigen-binding B cells (TNP-ABCs). The same TNP-ABCs (which had already processed TNP-keyhole limpet hemocyanin) formed conjugates with one or the other T helper (T(H)) cell type, and conjugation was antigen-specific and major histocompatibility complex-restricted. The conjugation of a fixed number of one type of T(H) cell to the TNP-ABCs was inhibited by co-incubation with increasing numbers of cells of the other T(H) cell type. Thus, the vast majority of TNP-ABCs can conjugate to either type of T(H) cell. A similar pattern of inhibition of the conjugation of one T(H) cell type to the TNP-ABCs was seen when cells were co-incubated with increasing numbers of cells from an alloreactive T-cell clone. In all cases, conjugates contained only one T cell bound to one B cell, suggesting that interaction of an antigen-presenting B cell with one T(H) cell prevents the simultaneous binding of another T(H) cell.
UR - http://www.scopus.com/inward/record.url?scp=0023692086&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023692086&partnerID=8YFLogxK
U2 - 10.1073/pnas.85.20.7724
DO - 10.1073/pnas.85.20.7724
M3 - Article
C2 - 2459715
AN - SCOPUS:0023692086
VL - 85
SP - 7724
EP - 7728
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 20
ER -