TY - JOUR
T1 - A small molecular activator of cardiac hypertrophy uncovered in a chemical screen for modifiers of the calcineurin signaling pathway
AU - Bush, Erik
AU - Fielitz, Jens
AU - Melvin, Lawrence
AU - Martinez-Arnold, Michael
AU - McKinsey, Timothy A.
AU - Plichta, Ryan
AU - Olson, Eric N.
PY - 2004/3/2
Y1 - 2004/3/2
N2 - The calcium, calmodulin-dependent phosphatase calcineurin, regulates growth and gene expression of striated muscles. The activity of calcineurin is modulated by a family of cofactors, referred to as modulatory calcineurin-interacting proteins (MCIPs). In the heart, the MCIP1 gene is activated by calcineurin and has been proposed to fulfill a negative feedback loop that restrains potentially pathological calcineurin signaling, which would otherwise lead to abnormal cardiac growth. In a high-throughput screen for small molecules capable of regulating MCIP1 expression in muscle cells, we identified a unique 4-aminopyridine derivative exhibiting an embedded partial structural motif of serotonin (5-hydroxytryptamine, 5-HT). This molecule, referred to as pyridine activator of myocyte hypertrophy, acts as a selective agonist for 5-HT2A/2B receptors and induces hypertrophy of cardiac muscle cells through a signaling pathway involving calcineurin and a kinase-dependent mechanism that inactivates class II histone deacetylases, which act as repressors of cardiac growth. These findings identify MCIP1 as a downstream target of 5-HT2A/2B receptor signaling in cardiac muscle cells and suggest possible uses for 5-HT2A/2B agonists and antagonists as modulators of cardiac growth and gene expression.
AB - The calcium, calmodulin-dependent phosphatase calcineurin, regulates growth and gene expression of striated muscles. The activity of calcineurin is modulated by a family of cofactors, referred to as modulatory calcineurin-interacting proteins (MCIPs). In the heart, the MCIP1 gene is activated by calcineurin and has been proposed to fulfill a negative feedback loop that restrains potentially pathological calcineurin signaling, which would otherwise lead to abnormal cardiac growth. In a high-throughput screen for small molecules capable of regulating MCIP1 expression in muscle cells, we identified a unique 4-aminopyridine derivative exhibiting an embedded partial structural motif of serotonin (5-hydroxytryptamine, 5-HT). This molecule, referred to as pyridine activator of myocyte hypertrophy, acts as a selective agonist for 5-HT2A/2B receptors and induces hypertrophy of cardiac muscle cells through a signaling pathway involving calcineurin and a kinase-dependent mechanism that inactivates class II histone deacetylases, which act as repressors of cardiac growth. These findings identify MCIP1 as a downstream target of 5-HT2A/2B receptor signaling in cardiac muscle cells and suggest possible uses for 5-HT2A/2B agonists and antagonists as modulators of cardiac growth and gene expression.
UR - http://www.scopus.com/inward/record.url?scp=1542297759&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1542297759&partnerID=8YFLogxK
U2 - 10.1073/pnas.0308723101
DO - 10.1073/pnas.0308723101
M3 - Article
C2 - 14976250
AN - SCOPUS:1542297759
SN - 0027-8424
VL - 101
SP - 2870
EP - 2875
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 9
ER -