A splice variant of the transcript for guanylyl cyclase C is expressed in human colon and colorectal cancer cells

Joshua M. Pearlman, Scott P. Prawer, Michael T. Barber, Scott J. Parkinson, Stephanie Schulz, Jason Park, Matt Zook, Scott A. Waldman

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Guanylyl cyclase C is a sensitive and specific biomarker for metastatic colorectal cancer. A variant of the guanylyl cyclase C transcript was identified that possesses a 142-bp deletion at the 3' end of exon 1 reflecting alternative splicing of mRNA, introducing a shift in the open reading frame that prevents translation of a guanylyl cyclase C-related product. This variant was identified in human intestine and colon carcinomas, but not in extraintestinal tissues or tumors. These studies demonstrate that GCC and the splice variant contribute to the pool of GCC transcripts detected by RT-PCR in human tissues. They indicate that primers for RT-PCR that amplify regions downstream from the deletion are required to assess the full complement of GCC transcripts (GCC + GCC(var)) in human tissues and body fluids for staging and postoperative surveillance of patients with colorectal cancer.

Original languageEnglish (US)
Pages (from-to)298-305
Number of pages8
JournalDigestive Diseases and Sciences
Volume45
Issue number2
DOIs
StatePublished - Mar 16 2000

Keywords

  • Alternate 5' splice acceptor sites
  • Colorectal cancer
  • Guanylyl cyclase C
  • Receptor transcript splice variant
  • Tumor marker

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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  • Cite this

    Pearlman, J. M., Prawer, S. P., Barber, M. T., Parkinson, S. J., Schulz, S., Park, J., Zook, M., & Waldman, S. A. (2000). A splice variant of the transcript for guanylyl cyclase C is expressed in human colon and colorectal cancer cells. Digestive Diseases and Sciences, 45(2), 298-305. https://doi.org/10.1023/A:1005456408768