A stainless protocol for high quality RNA isolation from laser capture microdissected purkinje cells in the human post-mortem cerebellum

Engineering & Materials Science

• RNA 69%
• Microdissection 69%
• Lasers 43%
• Tissue 36%
• Visualization 33%
• Ultraviolet lasers 25%
• Cells 14%
• Neurons 13%
• Brain 11%
• Xylene 9%
• Lipids 9%
• Reagents 8%
• Purification 8%
• Optical microscopy 7%
• Microscopic examination 7%
• DNA 7%
• Ethanol 7%
• Proteins 6%
• Experiments 2%

Medicine & Life Sciences

• Purkinje Cells 65%
• Cerebellum 53%
• Laser Capture Microdissection 49%
• Lasers 46%
• RNA 40%
• Microscopy 12%
• Xylenes 10%
• Staining and Labeling 9%
• Cerebellar Cortex 9%
• Neurons 8%
• Cell Body 7%
• Brain 7%
• Ethanol 6%
• Cell Count 5%
• Lipids 4%
• Phenotype 4%
• DNA 4%
• Proteins 2%

Chemical Compounds

• Laser Capture Microdissection 100%
• Xylene 20%
• Optical Microscopy 17%
• Lipid 13%
• Purification 13%
• Ethanol 12%
• Protein 7%
• Reaction Yield 7%