A strategy for founding a global lymphocyte proteinpaedia and gene catalogue

Ivan Lefkovits, John R. Kettman, Christopher Coleclough

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

In this article, Ivan Lefkovits, John Kettman and Christopher Coleclough describe a strategy for a comprehensive analysis of the genes active in lymphocytes. It allows direct access to the coding sequence of any gene, provided that the two-dimensional gel spot coordinate position is known. The authors have constructed representative lymphocyte cDNA libraries and partitioned them into many sectors, each containing a unique set of cDNA clones. Data from the analysis of natural lymphocyte protein is compiled into a 'proteinpaedia', which is matched to a gene catalogue comprising the partitioned recombinant phage collection.

Original languageEnglish (US)
Pages (from-to)157-162
Number of pages6
JournalImmunology Today
Volume11
Issue numberC
DOIs
StatePublished - 1990

Fingerprint

Lymphocytes
Genes
Gene Library
Bacteriophages
Complementary DNA
Clone Cells
Gels
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

A strategy for founding a global lymphocyte proteinpaedia and gene catalogue. / Lefkovits, Ivan; Kettman, John R.; Coleclough, Christopher.

In: Immunology Today, Vol. 11, No. C, 1990, p. 157-162.

Research output: Contribution to journalArticle

Lefkovits, Ivan ; Kettman, John R. ; Coleclough, Christopher. / A strategy for founding a global lymphocyte proteinpaedia and gene catalogue. In: Immunology Today. 1990 ; Vol. 11, No. C. pp. 157-162.
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