A Tagging-via-Substrate Technology for Genome-Wide Detection and Identification of Farnesylated Proteins

Sung Chan Kim, Yoonjung Kho, Deb Barma, J R Falck, Yingming Zhao

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Protein farnesylation is one of the most common lipid modifications and has an important role in the regulation of various cellular functions. We have recently developed a novel proteomics strategy, designated the tagging-via-substrate (TAS) approach, for the detection and proteomic analysis of farnesylated proteins. This chapter describes the principle of TAS technology and details the method for detection and enrichment of farnesylated proteins.

Original languageEnglish (US)
Pages (from-to)629-637
Number of pages9
JournalMethods in Enzymology
Volume407
DOIs
Publication statusPublished - 2005

    Fingerprint

ASJC Scopus subject areas

  • Biochemistry

Cite this