A Tagging-via-Substrate Technology for Genome-Wide Detection and Identification of Farnesylated Proteins

Sung Chan Kim; Yoonjung Kho; Deb Barma; J R Falck; Yingming Zhao

doi:10.1016/S0076-6879(05)07049-7

A Tagging-via-Substrate Technology for Genome-Wide Detection and Identification of Farnesylated Proteins

Sung Chan Kim, Yoonjung Kho, Deb Barma, J R Falck, Yingming Zhao

Biochemistry

Research output: Contribution to journal › Review article › peer-review

2 Scopus citations

Abstract

Protein farnesylation is one of the most common lipid modifications and has an important role in the regulation of various cellular functions. We have recently developed a novel proteomics strategy, designated the tagging-via-substrate (TAS) approach, for the detection and proteomic analysis of farnesylated proteins. This chapter describes the principle of TAS technology and details the method for detection and enrichment of farnesylated proteins.

Original language	English (US)
Pages (from-to)	629-637
Number of pages	9
Journal	Methods in Enzymology
Volume	407
DOIs	https://doi.org/10.1016/S0076-6879(05)07049-7
State	Published - Jan 1 2006

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/S0076-6879(05)07049-7

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abstract = "Protein farnesylation is one of the most common lipid modifications and has an important role in the regulation of various cellular functions. We have recently developed a novel proteomics strategy, designated the tagging-via-substrate (TAS) approach, for the detection and proteomic analysis of farnesylated proteins. This chapter describes the principle of TAS technology and details the method for detection and enrichment of farnesylated proteins.",

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AU - Chan Kim, Sung

AU - Kho, Yoonjung

AU - Barma, Deb

AU - Falck, J R

AU - Zhao, Yingming

PY - 2006/1/1

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N2 - Protein farnesylation is one of the most common lipid modifications and has an important role in the regulation of various cellular functions. We have recently developed a novel proteomics strategy, designated the tagging-via-substrate (TAS) approach, for the detection and proteomic analysis of farnesylated proteins. This chapter describes the principle of TAS technology and details the method for detection and enrichment of farnesylated proteins.

AB - Protein farnesylation is one of the most common lipid modifications and has an important role in the regulation of various cellular functions. We have recently developed a novel proteomics strategy, designated the tagging-via-substrate (TAS) approach, for the detection and proteomic analysis of farnesylated proteins. This chapter describes the principle of TAS technology and details the method for detection and enrichment of farnesylated proteins.

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JO - Methods in Enzymology

JF - Methods in Enzymology

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A Tagging-via-Substrate Technology for Genome-Wide Detection and Identification of Farnesylated Proteins

Abstract

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