A TATA sequence-dependent transcriptional repressor activity associated with mammalian transcription factor IIA

Teijiro Aso, Hiroaki Serizawa, Ronald C. Conaway, Joan Weliky Conaway

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

In the process of characterizing cellular proteins that modulate basal transcription by RNA polymerase II, we identified a novel repressor activity specific for promoters containing consensus TATA boxes. This activity strongly represses TATA-binding protein (TBP)-dependent transcription initiation from core promoter elements containing a consensus TATA sequence, but activates TBP-dependent transcription from core promoter elements lacking a consensus TATA sequence. Purification of this activity to near homogeneity from rat liver nuclear extracts led to the surprising discovery that it co-purifies closely with mammalian transcription factor IIA (TFIIA). The close association of TATA sequence-dependent transcriptional repressor activity with TFIIA adds a new and unexpected dimension to the already complex picture of this factor's function in transcription by RNA polymerase II.

Original languageEnglish (US)
Pages (from-to)435-445
Number of pages11
JournalEMBO Journal
Volume13
Issue number2
DOIs
StatePublished - Jan 15 1994
Externally publishedYes

Keywords

  • Core promoter
  • Holo-TFIID
  • RNA polymerase II
  • TBP
  • TFIIA

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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