A transcriptional network in polycystic kidney disease

Lionel Gresh, Evelyne Fischer, Andreas Reimann, Myriam Tanguy, Serge Garbay, Xinli Shao, Thomas Hiesberger, Laurence Fiette, Peter Igarashi, Moshe Yaniv, Marco Pontoglio

Research output: Contribution to journalArticle

241 Citations (Scopus)

Abstract

Mutations in cystic kidney disease genes represent a major genetic cause of end-stage renal disease. However, the molecular cascades controlling the expression of these genes are still poorly understood. Hepatocyte Nuclear Factor 1β (HNF1β) is a homeoprotein predominantly expressed in renal, pancreatic and hepatic epithelia. We report here that mice with renal-specific inactivation of HNF1β develop polycystic kidney disease. We show that renal cyst formation is accompanied by a drastic defect in the transcriptional activation of Umod, Pkhd1 and Pkd2 genes, whose mutations are responsible for distinct cystic kidney syndromes. In vivo chromatin immunoprecipitation experiments demonstrated that HNF1β binds to several DNA elements in murine Umod, Pkhd1, Pkd2 and Tg737/Polaris genomic sequences. Our results uncover a direct transcriptional hierarchy between HNF1β and cystic disease genes. Interestingly, most of the identified HNF1β target gene products colocalize to the primary cilium, a crucial organelle that plays an important role in controlling the proliferation of tubular cells. This may explain the increased proliferation of cystic cells in MODY5 patients carrying autosomal dominant mutations in HNF1β.

Original languageEnglish (US)
Pages (from-to)1657-1668
Number of pages12
JournalEMBO Journal
Volume23
Issue number7
DOIs
StatePublished - Apr 7 2004

Fingerprint

Hepatocyte Nuclear Factor 1
Polycystic Kidney Diseases
Gene Regulatory Networks
Genes
Cystic Kidney Diseases
Kidney
Mutation
Cell Proliferation
Homeodomain Proteins
Cilia
Chromatin Immunoprecipitation
Organelles
Transcriptional Activation
Chronic Kidney Failure
Chromatin
Cysts
Epithelium
Chemical activation
Gene Expression
Defects

Keywords

  • Cilium
  • Cysts
  • HNF1β
  • MODY5
  • Proliferation

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Gresh, L., Fischer, E., Reimann, A., Tanguy, M., Garbay, S., Shao, X., ... Pontoglio, M. (2004). A transcriptional network in polycystic kidney disease. EMBO Journal, 23(7), 1657-1668. https://doi.org/10.1038/sj.emboj.7600160

A transcriptional network in polycystic kidney disease. / Gresh, Lionel; Fischer, Evelyne; Reimann, Andreas; Tanguy, Myriam; Garbay, Serge; Shao, Xinli; Hiesberger, Thomas; Fiette, Laurence; Igarashi, Peter; Yaniv, Moshe; Pontoglio, Marco.

In: EMBO Journal, Vol. 23, No. 7, 07.04.2004, p. 1657-1668.

Research output: Contribution to journalArticle

Gresh, L, Fischer, E, Reimann, A, Tanguy, M, Garbay, S, Shao, X, Hiesberger, T, Fiette, L, Igarashi, P, Yaniv, M & Pontoglio, M 2004, 'A transcriptional network in polycystic kidney disease', EMBO Journal, vol. 23, no. 7, pp. 1657-1668. https://doi.org/10.1038/sj.emboj.7600160
Gresh L, Fischer E, Reimann A, Tanguy M, Garbay S, Shao X et al. A transcriptional network in polycystic kidney disease. EMBO Journal. 2004 Apr 7;23(7):1657-1668. https://doi.org/10.1038/sj.emboj.7600160
Gresh, Lionel ; Fischer, Evelyne ; Reimann, Andreas ; Tanguy, Myriam ; Garbay, Serge ; Shao, Xinli ; Hiesberger, Thomas ; Fiette, Laurence ; Igarashi, Peter ; Yaniv, Moshe ; Pontoglio, Marco. / A transcriptional network in polycystic kidney disease. In: EMBO Journal. 2004 ; Vol. 23, No. 7. pp. 1657-1668.
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