A transcriptionally active DNA-binding site for human p53 protein complexes

Walter D. Funk, Daniel T. Pak, Richard H. Karas, Woodring E. Wright, Jerry W. Shay

Research output: Contribution to journalArticlepeer-review

661 Scopus citations

Abstract

Recent studies have demonstrated transcriptional activation domains within the tumor suppressor protein p53, while others have described specific DNA- binding sites for p53, implying that the protein may act as a transcriptional regulatory factor. We have used a reiterative selection procedure (CASTing: cyclic amplification and selection of targets) to identify new specific binding sites for p53, using nuclear extracts from normal human fibroblasts as the source of p53 protein. The preferred consensus is the palindrome GGACATGCCCGGGCATGTCC. In vitro-translated p53 binds to this sequence only when mixed with nuclear extracts, suggesting that p53 may bind DNA after posttranslational modification or as a complex with other protein partners. When placed upstream of a reporter construct, this sequence promotes p53- dependent transcription in transient transfection assays.

Original languageEnglish (US)
Pages (from-to)2866-2871
Number of pages6
JournalMolecular and cellular biology
Volume12
Issue number6
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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