A transforming growth factor β1 receptor type II mutation in ulcerative colitis-associated neoplasms

R. F. Souza, J. Lei, J. Yin, R. Appel, T. T. Zou, X. Zhou, S. Wang, G. Rhyu M.-, K. Cymes, O. Chan, W. S. Park, M. J. Krasna, B. D. Greenwald, J. Cottrell, J. M. Abraham, L. Simms, B. Leggett, J. Young, N. Harpaz, S. J. Meltzer

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Abstract

Background and Aims: Numerous gastrointestinal tumors, notably sporadic and ulcerative colitis (UC)-associated colorectal carcinomas and dysplasias, gastric cancers, and esophageal carcinomas, manifest microsatellite instability. Recently, a transforming growth factor β1 type II receptor (TGF-β1RII) mutation in a coding microsatellite was described in colorectal carcinomas showing instability. One hundred thirty-eight human neoplasms (61 UC-associated, 35 gastric, 26 esophageal, and 16 sporadic colorectal) were evaluated for this TGF-β1RII mutation. Methods: Whether instability was present at other chromosomal loci in these lesions was determined. In lesions manifesting or lacking instability, the TGF-β1RII coding region polydeoxyadenine (poly A) microsatellite tract was polymerase chain reaction amplified with 32P-labeled deoxycytidine triphosphate. Polymerase chain reaction products were electrophoresed on denaturing gels and exposed to radiographic film. Results: Three of 18 UC specimens with instability at other chromosomal loci (17%) showed TGF-β1RII poly A tract mutation, including 2 cancers and 1 dysplasia; moreover, 2% of UC specimens without instability (1 of 43) (1 cancer), 81% of unstable sporadic colorectal cancers (13 of 16), and none of the 61 stable or unstable gastric or esophageal cancers contained TGF-β1RII mutations. Conclusions: Mutational inactivation of the poly A microsatellite tract within TGF-β1RII occurs early and in a subset of unstable UC neoplasms and commonly in sporadic colorectal cancers but may be rare in unstable gastric and esophageal tumors.

Original languageEnglish (US)
Pages (from-to)40-45
Number of pages6
JournalGastroenterology
Volume112
Issue number1
DOIs
StatePublished - Jan 1 1997

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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    Souza, R. F., Lei, J., Yin, J., Appel, R., Zou, T. T., Zhou, X., Wang, S., Rhyu M.-, G., Cymes, K., Chan, O., Park, W. S., Krasna, M. J., Greenwald, B. D., Cottrell, J., Abraham, J. M., Simms, L., Leggett, B., Young, J., Harpaz, N., & Meltzer, S. J. (1997). A transforming growth factor β1 receptor type II mutation in ulcerative colitis-associated neoplasms. Gastroenterology, 112(1), 40-45. https://doi.org/10.1016/S0016-5085(97)70217-8