A Twist2-dependent progenitor cell contributes to adult skeletal muscle

Ning Liu, Glynnis A. Garry, Stephen Li, Svetlana Bezprozvannaya, Efrain Sanchez-Ortiz, Beibei Chen, John M. Shelton, Priscilla Jaichander, Rhonda Bassel-Duby, Eric N. Olson

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Skeletal muscle possesses remarkable regenerative potential due to satellite cells, an injury-responsive stem cell population located beneath the muscle basal lamina that expresses Pax7. By lineage tracing of progenitor cells expressing the Twist2 (Tw2) transcription factor in mice, we discovered a myogenic lineage that resides outside the basal lamina of adult skeletal muscle. Tw2+ progenitors are molecularly and anatomically distinct from satellite cells, are highly myogenic in vitro, and can fuse with themselves and with satellite cells. Tw2+ progenitors contribute specifically to type IIb/x myofibres during adulthood and muscle regeneration, and their genetic ablation causes wasting of type IIb myofibres. We show that Tw2 expression maintains progenitor cells in an undifferentiated state that is poised to initiate myogenesis in response to appropriate cues that extinguish Tw2 expression. Tw2-expressing myogenic progenitors represent a previously unrecognized, fibre-type-specific stem cell involved in postnatal muscle growth and regeneration.

Original languageEnglish (US)
Pages (from-to)202-213
Number of pages12
JournalNature Cell Biology
Volume19
Issue number3
DOIs
StatePublished - Mar 1 2017

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Skeletal Muscle
Stem Cells
Basement Membrane
Muscles
Regeneration
Skeletal Muscle Satellite Cells
Muscle Development
Cues
Transcription Factors
Wounds and Injuries
Growth
Population

ASJC Scopus subject areas

  • Cell Biology

Cite this

Liu, N., Garry, G. A., Li, S., Bezprozvannaya, S., Sanchez-Ortiz, E., Chen, B., ... Olson, E. N. (2017). A Twist2-dependent progenitor cell contributes to adult skeletal muscle. Nature Cell Biology, 19(3), 202-213. https://doi.org/10.1038/ncb3477

A Twist2-dependent progenitor cell contributes to adult skeletal muscle. / Liu, Ning; Garry, Glynnis A.; Li, Stephen; Bezprozvannaya, Svetlana; Sanchez-Ortiz, Efrain; Chen, Beibei; Shelton, John M.; Jaichander, Priscilla; Bassel-Duby, Rhonda; Olson, Eric N.

In: Nature Cell Biology, Vol. 19, No. 3, 01.03.2017, p. 202-213.

Research output: Contribution to journalArticle

Liu, N, Garry, GA, Li, S, Bezprozvannaya, S, Sanchez-Ortiz, E, Chen, B, Shelton, JM, Jaichander, P, Bassel-Duby, R & Olson, EN 2017, 'A Twist2-dependent progenitor cell contributes to adult skeletal muscle', Nature Cell Biology, vol. 19, no. 3, pp. 202-213. https://doi.org/10.1038/ncb3477
Liu N, Garry GA, Li S, Bezprozvannaya S, Sanchez-Ortiz E, Chen B et al. A Twist2-dependent progenitor cell contributes to adult skeletal muscle. Nature Cell Biology. 2017 Mar 1;19(3):202-213. https://doi.org/10.1038/ncb3477
Liu, Ning ; Garry, Glynnis A. ; Li, Stephen ; Bezprozvannaya, Svetlana ; Sanchez-Ortiz, Efrain ; Chen, Beibei ; Shelton, John M. ; Jaichander, Priscilla ; Bassel-Duby, Rhonda ; Olson, Eric N. / A Twist2-dependent progenitor cell contributes to adult skeletal muscle. In: Nature Cell Biology. 2017 ; Vol. 19, No. 3. pp. 202-213.
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