A unique cell-surface protein phenotype distinguishes human small-cell from non-small-cell lung cancer.

S. B. Baylin, A. F. Gazdar, J. D. Minna, S. D. Bernal, J. H. Shaper

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Abstract

We have used radioiodination (125I) and two-dimensional polyacrylamide gel electrophoresis to determine that small- (oat) cell lung carcinoma (SCC)--a tumor with neuroendocrine features--possesses a surface protein pattern distinct from the other types of lung cancer cells (squamous, adeno-, and large-cell undifferentiated carcinoma). Twelve distinguishing proteins, 40 to 70 kilodaltons (kDal), characterized four separate lines of SCC; three of these, designated E (60 kDal; pI = 7.3), S (30 kDal; pI = 6.0), and U (57 kDal; pI = 5.6), may be unique SCC gene products and were identified only in [35S]methionine labeling of SCC and not in non-SCC or human fibroblasts. Two lines of adeno-, one of squamous, and one of undifferentiated large-cell lung carcinoma exhibited similar surface protein patterns to one another. Nine distinguishing proteins (40 to 100 kDal) and at least five large proteins (greater than 100 kDal) were unique to these lines. The surface protein phenotypes for SCC and non-SCC were distinct from those for human lymphoblastoid cells and fibroblasts. However, the neuroendocrine features of SCC were further substantiated because 6 of the 12 distinguishing SCC surface proteins, including E and U, were identified on human neuroblastoma cells. The proteins identified should (i) help define differentiation steps for normal and neoplastic bronchial epithelial cells, (ii) prove useful in better classifying lung cancers, and (iii) be instrumental in tracing formation of neuroendocrine cells.

Original languageEnglish (US)
Pages (from-to)4650-4654
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number15
StatePublished - Aug 1982

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Non-Small Cell Lung Carcinoma
Membrane Proteins
Phenotype
Large Cell Carcinoma
Lung Neoplasms
Proteins
Fibroblasts
Epithelial Cells
Neuroendocrine Cells
Small Cell Carcinoma
Neuroendocrine Tumors
Small Cell Lung Carcinoma
Electrophoresis, Gel, Two-Dimensional
Neuroblastoma
Methionine
Carcinoma
Lung
Genes

ASJC Scopus subject areas

  • General
  • Genetics

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A unique cell-surface protein phenotype distinguishes human small-cell from non-small-cell lung cancer. / Baylin, S. B.; Gazdar, A. F.; Minna, J. D.; Bernal, S. D.; Shaper, J. H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, No. 15, 08.1982, p. 4650-4654.

Research output: Contribution to journalArticle

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