A unique variant of a homeobox gene related to Drosophila cut is expressed in mouse testis

Gregory B. Vanden Heuvel, Susan E. Quaggin, Peter Igarashi

Research output: Contribution to journalArticle

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Abstract

Mammalian homologues of the Drosophila cut homeobox gene encode transcriptional repressors that are involved in tissue-specific and developmental gene regulation. We examined the expression of a murine cut homologue (Cux-1) in the adult mouse. In many somatic tissues, Cux-1 was expressed as a 13-kb transcript. However, the highest expression of Cux-1 was in the testis, where a unique 2.4-kb splice variant was identified. Less abundant transcripts of 5 kb, 6.5 kb, and 8.5 kb were also detected only in the testis. The nucleotide sequence of the 2.4-kb Cux-1 transcript was identical to the 13-kb transcript in the region of overlap, but the testis- specific transcript encoded a truncated protein that contained only one Cut repeat in addition to the Cut-related homeodomain. Studies of mice homozygous for the atrichosis (at/at) mutation suggested that the 2.4-kb transcript was expressed in germ cells in the testis. In situ hybridization verified that Cux-1 was transiently expressed in postmeiotic germ cells at the round spermatid stage. Immunoblot analysis of nuclear extracts showed that the testis-specific Cux-1 transcripts encoded a 55-kDa protein. These results demonstrate that multiple products of a cut-related homeobox gene are expressed in the testis. The highly restricted pattern of expression of Cux- 1 in the testis suggests that it may be involved in regulation of postmeiotic gene expression.

Original languageEnglish (US)
Pages (from-to)731-739
Number of pages9
JournalBiology of Reproduction
Volume55
Issue number4
StatePublished - Oct 1996

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Homeobox Genes
Drosophila
Testis
Germ Cells
Developmental Genes
Spermatids
Gene Expression Regulation
In Situ Hybridization
Proteins
Mutation

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Vanden Heuvel, G. B., Quaggin, S. E., & Igarashi, P. (1996). A unique variant of a homeobox gene related to Drosophila cut is expressed in mouse testis. Biology of Reproduction, 55(4), 731-739.

A unique variant of a homeobox gene related to Drosophila cut is expressed in mouse testis. / Vanden Heuvel, Gregory B.; Quaggin, Susan E.; Igarashi, Peter.

In: Biology of Reproduction, Vol. 55, No. 4, 10.1996, p. 731-739.

Research output: Contribution to journalArticle

Vanden Heuvel, GB, Quaggin, SE & Igarashi, P 1996, 'A unique variant of a homeobox gene related to Drosophila cut is expressed in mouse testis', Biology of Reproduction, vol. 55, no. 4, pp. 731-739.
Vanden Heuvel, Gregory B. ; Quaggin, Susan E. ; Igarashi, Peter. / A unique variant of a homeobox gene related to Drosophila cut is expressed in mouse testis. In: Biology of Reproduction. 1996 ; Vol. 55, No. 4. pp. 731-739.
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