A variant of DNA polymerase β is not cancer specific

Dawei Bu, Leslie R. Cler, Cheryl M. Lewis, David M. Euhus

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

DNA Polymerase β (Pol β) carries out base-excision repair (BER) required for DNA maintenance, replication, and recombination in eukaryotic cells. A variant characterized by a deletion of exon 11, an 87-bp region in the catalytic domain (pol βΔ208-236), was previously described as a possible cause of genomic instability in cancer. The variant form was hypothesized to act in a dominant negative fashion, due to the fact that the variant inhibits the gap filling and DNA binding activities of the wild-type pol β protein. DNA polymerase β transcripts were analyzed in 8 breast cancer cell lines, snap-frozen benign breast tissues from 10 women, and lymphocytes from 10 normal controls, using reverse-transcription polymerase chain reaction (RT-PCR) and three separate primer pairs. The exon 10-12 splice site (variant) was identified using a primer designed to span the spliced exons and by sequencing RT-PCR products that included exon 10, exon 11 (if present), and exon 12. In all of the samples tested, we found both the wild-type and exon 11 87-bp deleted variant mRNAs expressed. We conclude that expression of the DNA polymerase β variant (pol βΔ208-236) is ubiquitous and not breast cancer specific.

Original languageEnglish (US)
Pages (from-to)327-331
Number of pages5
JournalJournal of Investigative Surgery
Volume17
Issue number6
DOIs
StatePublished - Nov 2004

Fingerprint

DNA-Directed DNA Polymerase
Exons
Neoplasms
Reverse Transcription
pol Gene Products
Breast Neoplasms
Polymerase Chain Reaction
Genomic Instability
Eukaryotic Cells
DNA Replication
DNA Repair
Genetic Recombination
Catalytic Domain
Breast
Maintenance
Lymphocytes
Cell Line
Messenger RNA
DNA

Keywords

  • Breast cancer
  • DNA polymerase β
  • Polymerase β δ208-236

ASJC Scopus subject areas

  • Surgery

Cite this

A variant of DNA polymerase β is not cancer specific. / Bu, Dawei; Cler, Leslie R.; Lewis, Cheryl M.; Euhus, David M.

In: Journal of Investigative Surgery, Vol. 17, No. 6, 11.2004, p. 327-331.

Research output: Contribution to journalArticle

Bu, Dawei ; Cler, Leslie R. ; Lewis, Cheryl M. ; Euhus, David M. / A variant of DNA polymerase β is not cancer specific. In: Journal of Investigative Surgery. 2004 ; Vol. 17, No. 6. pp. 327-331.
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