TY - JOUR
T1 - A Vibrio effector protein is an inositol phosphatase and disrupts host cell membrane integrity
AU - Broberg, Christopher A.
AU - Zhang, Lingling
AU - Gonzalez, Herman
AU - Laskowski-Arce, Michelle A.
AU - Orth, Kim
PY - 2010/9/24
Y1 - 2010/9/24
N2 - The marine bacterium Vibrio parahaemolyticus causes gastroenteritis in humans and encodes the type III effector protein VPA0450, which contributes to host cell death caused by autophagy, cell rounding, and cell lysis. We found that VPA0450 is an inositol polyphosphate 5-phosphatase that hydrolyzed the D5 phosphate from the plasma membrane phospholipid phosphatidylinositol 4,5-bisphosphate. VPA0450 disrupted cytoskeletal binding sites on the inner surface of membranes of human cells and caused plasma membrane blebbing, which compromised membrane integrity and probably contributed to cell death by facilitating lysis. Thus, bacterial pathogens can disrupt adaptor protein-binding sites required for proper membrane and cytoskeleton dynamics by altering the homeostasis of membrane-bound inositol-signaling molecules.
AB - The marine bacterium Vibrio parahaemolyticus causes gastroenteritis in humans and encodes the type III effector protein VPA0450, which contributes to host cell death caused by autophagy, cell rounding, and cell lysis. We found that VPA0450 is an inositol polyphosphate 5-phosphatase that hydrolyzed the D5 phosphate from the plasma membrane phospholipid phosphatidylinositol 4,5-bisphosphate. VPA0450 disrupted cytoskeletal binding sites on the inner surface of membranes of human cells and caused plasma membrane blebbing, which compromised membrane integrity and probably contributed to cell death by facilitating lysis. Thus, bacterial pathogens can disrupt adaptor protein-binding sites required for proper membrane and cytoskeleton dynamics by altering the homeostasis of membrane-bound inositol-signaling molecules.
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U2 - 10.1126/science.1192850
DO - 10.1126/science.1192850
M3 - Article
C2 - 20724587
AN - SCOPUS:77957149833
SN - 0036-8075
VL - 329
SP - 1660
EP - 1662
JO - Science
JF - Science
IS - 5999
ER -