A Vibrio effector protein is an inositol phosphatase and disrupts host cell membrane integrity

Christopher A. Broberg, Lingling Zhang, Herman Gonzalez, Michelle A. Laskowski-Arce, Kim Orth

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The marine bacterium Vibrio parahaemolyticus causes gastroenteritis in humans and encodes the type III effector protein VPA0450, which contributes to host cell death caused by autophagy, cell rounding, and cell lysis. We found that VPA0450 is an inositol polyphosphate 5-phosphatase that hydrolyzed the D5 phosphate from the plasma membrane phospholipid phosphatidylinositol 4,5-bisphosphate. VPA0450 disrupted cytoskeletal binding sites on the inner surface of membranes of human cells and caused plasma membrane blebbing, which compromised membrane integrity and probably contributed to cell death by facilitating lysis. Thus, bacterial pathogens can disrupt adaptor protein-binding sites required for proper membrane and cytoskeleton dynamics by altering the homeostasis of membrane-bound inositol-signaling molecules.

Original languageEnglish (US)
Pages (from-to)1660-1662
Number of pages3
JournalScience
Volume329
Issue number5999
DOIs
StatePublished - Sep 24 2010

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Vibrio
Cell Membrane
Membranes
Cell Death
Binding Sites
Vibrio parahaemolyticus
Proteins
Autophagy
Gastroenteritis
Inositol
Blister
Phosphatidylinositols
Cytoskeleton
Protein Binding
Phospholipids
Homeostasis
Phosphates
Bacteria
myo-inositol-1 (or 4)-monophosphatase

ASJC Scopus subject areas

  • General

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A Vibrio effector protein is an inositol phosphatase and disrupts host cell membrane integrity. / Broberg, Christopher A.; Zhang, Lingling; Gonzalez, Herman; Laskowski-Arce, Michelle A.; Orth, Kim.

In: Science, Vol. 329, No. 5999, 24.09.2010, p. 1660-1662.

Research output: Contribution to journalArticle

Broberg, CA, Zhang, L, Gonzalez, H, Laskowski-Arce, MA & Orth, K 2010, 'A Vibrio effector protein is an inositol phosphatase and disrupts host cell membrane integrity', Science, vol. 329, no. 5999, pp. 1660-1662. https://doi.org/10.1126/science.1192850
Broberg, Christopher A. ; Zhang, Lingling ; Gonzalez, Herman ; Laskowski-Arce, Michelle A. ; Orth, Kim. / A Vibrio effector protein is an inositol phosphatase and disrupts host cell membrane integrity. In: Science. 2010 ; Vol. 329, No. 5999. pp. 1660-1662.
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