A yeast excision-repair gene is inducible by DNA damaging agents

G. W. Robinson, C. M. Nicolet, D. Kalainov, E. C. Friedberg

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Plasmids containing various RAD-lacZ gene fusions were integrated into the chromosome of haploid yeast cells. These integrant strains were tested for expression of Escherichia coli β-galactosidase after treatment with agents that damage DNA or interfere with normal DNA replication. We did not observe induction of single-copy RAD1-lacZ or RAD3-lacZ fusion genes under the experimental conditions used. However, exposure of cell containing an integrated RAD2-lacZ fusion gene to UV-radiation, γ-radiation, 4-nitroquinoline 1-oxide, or nalidixic acid resulted in 4- to 6-fold enhanced expression of β-galactosidase. Induction of the RAD2 gene after treatment of untransformed cells with 4-nitroquinoline 1-oxide was confirmed by direct examination of RAD2 mRNA. Lower levels of induction (≃50%) were observed after treatment of cells with other chemicals. Induction of the RAD2-lacZ fusion gene was also observed in cells transformed with single-copy and multicopy autonomously replicating plasmids.

Original languageEnglish (US)
Pages (from-to)1842-1846
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number6
StatePublished - 1986

Fingerprint

Lac Operon
DNA Repair
Yeasts
Galactosidases
4-Nitroquinoline-1-oxide
DNA
Genes
Plasmids
Radiation
Nalidixic Acid
Gene Fusion
Haploidy
DNA Replication
DNA Damage
Chromosomes
Escherichia coli
Messenger RNA

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

A yeast excision-repair gene is inducible by DNA damaging agents. / Robinson, G. W.; Nicolet, C. M.; Kalainov, D.; Friedberg, E. C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 6, 1986, p. 1842-1846.

Research output: Contribution to journalArticle

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abstract = "Plasmids containing various RAD-lacZ gene fusions were integrated into the chromosome of haploid yeast cells. These integrant strains were tested for expression of Escherichia coli β-galactosidase after treatment with agents that damage DNA or interfere with normal DNA replication. We did not observe induction of single-copy RAD1-lacZ or RAD3-lacZ fusion genes under the experimental conditions used. However, exposure of cell containing an integrated RAD2-lacZ fusion gene to UV-radiation, γ-radiation, 4-nitroquinoline 1-oxide, or nalidixic acid resulted in 4- to 6-fold enhanced expression of β-galactosidase. Induction of the RAD2 gene after treatment of untransformed cells with 4-nitroquinoline 1-oxide was confirmed by direct examination of RAD2 mRNA. Lower levels of induction (≃50{\%}) were observed after treatment of cells with other chemicals. Induction of the RAD2-lacZ fusion gene was also observed in cells transformed with single-copy and multicopy autonomously replicating plasmids.",
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AB - Plasmids containing various RAD-lacZ gene fusions were integrated into the chromosome of haploid yeast cells. These integrant strains were tested for expression of Escherichia coli β-galactosidase after treatment with agents that damage DNA or interfere with normal DNA replication. We did not observe induction of single-copy RAD1-lacZ or RAD3-lacZ fusion genes under the experimental conditions used. However, exposure of cell containing an integrated RAD2-lacZ fusion gene to UV-radiation, γ-radiation, 4-nitroquinoline 1-oxide, or nalidixic acid resulted in 4- to 6-fold enhanced expression of β-galactosidase. Induction of the RAD2 gene after treatment of untransformed cells with 4-nitroquinoline 1-oxide was confirmed by direct examination of RAD2 mRNA. Lower levels of induction (≃50%) were observed after treatment of cells with other chemicals. Induction of the RAD2-lacZ fusion gene was also observed in cells transformed with single-copy and multicopy autonomously replicating plasmids.

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