A yeast heterogeneous nuclear ribonucleoprotein complex associated with RNA polymerase II

Nicholas K. Conrad, Scott M. Wilson, Eric J. Steinmetz, Meera Patturajan, David A. Brow, Maurice S. Swanson, Jeffry L. Corden

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

Recent evidence suggests a role for the carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II (pol II) in pre-mRNA processing. The yeast NRD1 gene encodes an essential RNA-binding protein that shares homology with mammalian CTD-binding proteins and is thought to regulate mRNA abundance by binding to a specific cis-acting element. The present work demonstrates genetic and physical interactions among Nrd1p, the pol II CTD, Nab3p, and the CTD kinase CTDK-I. Previous studies have shown that Nrd1p associates with the CTD of pol II in yeast two-hybrid assays via its CTD- interaction domain (CID). We show that nrd1 temperature-sensitive alleles are synthetically lethal with truncation of the CTD to 9 or 10 repeats. Nab3p, a yeast hnRNP, is a high-copy suppressor of some nrd1 temperature-sensitive alleles, interacts with Nrd1p in a yeast two-hybrid assay, and coimmunoprecipitates with Nrd1p. Temperature-sensitive alleles of NAB3 are suppressed by deletion of CTK1, a kinase that has been shown to phosphorylate the CTD and increase elongation efficiency in vitro. This set of genetic and physical interactions suggests a role for yeast RNA-binding proteins in transcriptional regulation.

Original languageEnglish (US)
Pages (from-to)557-571
Number of pages15
JournalGenetics
Volume154
Issue number2
StatePublished - Feb 1 2000

ASJC Scopus subject areas

  • Genetics

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    Conrad, N. K., Wilson, S. M., Steinmetz, E. J., Patturajan, M., Brow, D. A., Swanson, M. S., & Corden, J. L. (2000). A yeast heterogeneous nuclear ribonucleoprotein complex associated with RNA polymerase II. Genetics, 154(2), 557-571.