Aberrant methylation of heparan sulfate glucosamine 3-O-Sulfotransferase 2 genes as a biomarker in colorectal cancer

Yasuharu Tokuyama, Takao Takahashi, Naoki Okumura, Kenichi Nonaka, Yoshihiro Kawaguchi, Kazuya Yamaguchi, Shinji Osada, Adi Gazdar, Kazuhiro Yoshida

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Aim: The first aim was to determine whether hypermethylation of certain tumor related genes, including 30ST2, CHFR, RUNX3, and p16, could be detected in Japanese colorectal cancer (CRC) patients. The second was to utilize the most frequently hypermethylated genes as biomarkers for early detection of CRC. Materials and Methods: We investigated the aberrant promoter methylation profile of 4 genes in 129 colorectal tumors and corresponding normal mucosa. For the second step, bowel lavage and blood of the main tumor drainage vein from 21 CRC patients were analyzed. Results: The 30ST2 gene was the most frequently methylated (57%) and was methylated frequently (9/21, 43%) in bowel lavage but rarely (1/21; 4.7%) in the venous drainage. The concordance between 30ST2 methylation in bowel lavage and corresponding tumor samples was 71.4%. Conclusion: These results may show the possibility of early detection for CRC by examining 30ST2 gene methylation status in DNA extracted from stool.

Original languageEnglish (US)
Pages (from-to)4811-4818
Number of pages8
JournalAnticancer Research
Volume30
Issue number12
Publication statusPublished - Dec 2010

    Fingerprint

Keywords

  • 30ST2
  • Blood of main drainage vein
  • Bowel lavage
  • Early detection

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tokuyama, Y., Takahashi, T., Okumura, N., Nonaka, K., Kawaguchi, Y., Yamaguchi, K., ... Yoshida, K. (2010). Aberrant methylation of heparan sulfate glucosamine 3-O-Sulfotransferase 2 genes as a biomarker in colorectal cancer. Anticancer Research, 30(12), 4811-4818.